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작성일 : 20-08-17
[HansaBioMed Life Sciences] EV-Spinner, ultrafiltration concentrator MWCO 100 kDa

 

EV-Spinner, ultrafiltration concentrator MWCO 100 kDa

 

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EV-Spinner is a 2.5 ml non-stick ultrafiltration (UF) concentrator to enhance the recovery of extracellular

vesicle (EVs) during the concentration or buffer exchange step. The ultrafiltration works in the opposite

direction to the centrifugal force, providing higher particle recovery. The low protein binding membrane

(polyethersulphone) reduces the EV loss, compared to V-shape concetrators, and the reverse design of the

EV-spinner ensures that the filter does not clog.

 

The EV-Spinner is the perfect solution for concentrating small volumes of samples, lower than 2.5 ml (up to

2.5 ml the sample can be concentrated with the TFF-Easy).

 

Main advantages compared to the V-shape concentrators

• Non-stick

• Filter membrane (polyethersulphone) is low binding compared to the V-shape concentrators that usually

  use cellulose or nitrocellulose.

• Non-clogging. The filter is reusable more times than V-concentrators. The reusability depends on the

  sample used.

• Easy recovery of the retentate.  

 

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Sample type:

 

Fluids containing extracellular vesicles (EVs), exosomes and nanoparticles

Filter membrane:

 

Polyethersulphone

Plastic construction:

 

Polystyrene

Maximum sample volume:

 

2.5 ml

Hold-up volume:

 

0.050 ml

Centrifugation speed:

 

2500 g Swing bucket rotor

 

 

2000 g Fixed angle rotor

Shelf life:

 

24 months

  

Applications

• Concentration of solutions containing EVs, exosomes or nanoparticles.

• Concentration of diluted matrices as cell media or urine prior to EV isolation.

• Removal of small molecules and unbound dyes from solution containing EVs/exosomes and nanoparticles.

• EV and exosomes dialysis.

• Concentration of EV suspensions post SEC isolation.

 

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Ordering informations

     

Catalog No.

Product Name

Size

HBM-EVS100-24

EV-Spinner, ultrafiltration concentrator MWCO 100 kDa

24 concentrators

HBM-EVS100-48

48 concentrators

 

 

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