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작성일 : 20-04-13
[Profoldin] PncB Assays
Bacterial nicotinate phosphoribosyltransferase (PncB) Assays

 

Bacterial nicotinate phosphoribosyltransferase (PncB) Assays

    

Nicotinate phosphoribosyltransferase (NAPRTase or PncB) provides a pathway of regenerating nicotinic acid

adenine dinucleotide (NAD) through synthesizing nicotinic acid mononucleotide (NAMN). The enzyme

transfers nicotinate (NA) onto phosphoribosyl pyrophosphate (PRPP) releasing pyrophosphate. PNCB is

drug target for gram (+) bacteria.

 

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The Bacterial Nicotinate Phosphoribosyltransferase (PncB) Assay is based on measurement of the

pyrophosphate generated from the enzyme reaction.  The pyrophosphate is converted to phosphate by

pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay reactions and

detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can

be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay

can be used for screening inhibitors of PNCB in drug discovery research.  It may also be used for

characterization of PNCB enzyme.  

 

   

Ordering information

   

Catalog No.

Product Name

Size

PNCB100KN

S. pneumoniae PncB Assay Kit Plus-100

100 assays

PNCB500KN

S. pneumoniae PncB Assay Kit Plus-500

500 assays

PNCB100KS

S. aureus PncB Assay Kit Plus-100

100 assays

PNCB500KS

S. aureus PncB Assay Kit Plus-500

500 assays

 

 

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