Biotech Support Group
 
작성일 : 20-02-15
[Biotech Support Group] HemogloBind™ Nucleic Acid
Suspension Reagent to remove Hemoglobin interference for PCR

 

HemogloBind™ Nucleic Acid

Suspension Reagent to remove Hemoglobin interference for PCR

 

Some infectious viruses are known or suspected to be associated with red cells, such as Zika and West Nile

viruses. So to detect their presence, it is becoming increasingly apparent to use whole blood, rather than

plasma alone, as a starting sample type. However, the presence of hemoglobin from whole blood lysates is

challenging for PCR because of the large amount of hemoglobin present. HemogloBind™ Nucleic Acid is

designed to efficiently remove hemoglobin whilemaintaining very high recovery of nucleic acids, both RNA

and DNA.

 

Features;

• Suitable for infectious disease research or rigorous analysis for whole blood or heavily hemolyzed

  samples

• Scaleable suspension reagent

• Robust and simple, no phenol/chloroform or ammonium sulfate precipitation

• High Yield – free and soluble DNA or RNA is highly recoverable

• > 95% Hemoglobin and Protein removal from whole blood lysates & dried blood cards

• Eliminates inhibitory effects of heme for PCR applications

• Compatible with silica-type final isolation preps

 

 

RNA / DNA Recoverable from Blood

    

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

 

Effect of HemogloBind™ Nucleic Acid on RNA / DNA

  

Absorbance at 260nm showing DNA (250 μg/ml) in sample before and

after treatment with HemogloBind™ Nucleic Acid Suspension Reagent

(H0250)

  

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

   

Absorbance at 260nm showing RNA (500 μg /ml) in sample before and

after treatment with HemogloBind™ Nucleic Acid

  

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

 

Effect of HemogloBind™ Nucleic Acid on Lysed Blood

  

Absorbance at 410nm showing hemoglobin in blood before and

after treatment with HemogloBind™ Nucleic Acid Suspension

Reagent (H0250)

  

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

  

Absorbance at 280nm showing protein in blood before and after

treatment with HemogloBind™ Nucleic Acid Suspension Reagent

(H0250)

  

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

 

Product sheet (PDF)

 

 

Ordering informations

    

Catalog No.

Product Name

Size

H0250-20

HemogloBind™ Nucleic Acid

20 preps

H0250-200

HemogloBind™ Nucleic Acid

200 preps

   

 

▣ 관련 페이지 ; Biotech Support Group