HemogloBind™ Blood Card Reagent Kit

Hemoglobin depletion and protein enrichment from dried blood spots

Hemoglobin is a common contaminant from dried whole blood cards and not normally found in

serum samples. The HemogloBind™ Blood Card protocol was designed to substantially

reduce the presence of hemoglobin and its associated interference with many serum proteins

and other analytes.

HemogloBind™ is engineered for a high degree of selectivity and does not cross react with

most common serum components, making it an excellent sample prep in numerous

applications. These include analytical protocols where optical interference is problematic, such

as bilirubin and cholinesterase analysis.

Features;

• Dried blood spots are useful for low volume analyses, and simple collection and transport

• Protocols suitable for inexpensive whole blood card systems, no need for cell separation

• Hemoglobin binding >90%, with 30-45 minute spin-filter format

• Protocols based on ≤10 µl whole blood applied, but suspension format is flexible to most

  volumes

• Blood proteins and enzymes are enriched for biomarker and proteomic investigations.

• Removes hemoglobin from diverse species including human, sheep, bovine, goat, rat,

  mouse, etc.

• High throughput and scaleable.

Flow chart of HemogloBind™ Blood Card Application

SDS PAGE showing comparison of hemoglobin removal from blood card kit and

whole blood lysate

Absorbance at 410nm shows presence of hemoglobin

Product sheet (PDF)