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작성일 : 19-10-08
[Invent Biotechnologies, Inc.] Plant Cytosolic and Nuclear Protein Isolation Kit
Only need 150 mg of starting materials

 

Minute™ Plant Cytosolic and Nuclear Protein Isolation Kit

Only need 150 mg of starting materials

 

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               Compare with leading Plant Nuclei Isolation Kit (Click)

 

The kit is designed for rapid fractionation of fresh/frozen soft plant tissues, especially leaves. Unlike other

commercial kit and Lab protocols, of which large amount of starting material (a few grams) and lengthy

processing time are required, this kit only uses 100-200 mg starting material.  By using a specialized filter

cartridge coupled with our proprietary buffer system, four subcellular fractions (cytosol,nuclei, chloroplasts

and organelles) can be obtained in about 1 hour. Thesubcellular fractions can be used in numerous

applications that include but not limited to protein trafficking, nuclear protein extraction and nucleic acid

purification/sequencing. The highly enriched nuclei can be used for proteomics, flow cytometry analysis,

DNA sequencing, and protein-protein/protein-DNA interaction studies. The subcellular fractionation protocol

includes grinding tissue to release plant cells followed by disruption of the cell membrane by rapidly passing

through the filter. Different subcellularfraction is obtained by differential centrifugation using only a table top

centrifuge. This kit has been tested for use with many species such as Arabidopsis, Spinach, Peas, and

Rape.

 

    클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

    클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

  클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

 

Instruction sheet (PDF)

 

 

Ordering information

     

Catalog No

Product Name

Kit components

Amoun

PF-045

Minute™ Plant Cytosolic and Nuclear Protein Isolation Kit

Buffer A

30 ml

Buffer B

1 ml

Buffer C

25 ml

Filter Cartridges with Collection Tubes

20 units

Collection Tubes with Caps

50 units

Pestle for 1.5 ml Eppendorf tubes

2 units

Protein Extraction Power

2 g

* For 20 preps

 

 

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