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작성일 : 19-05-25
[HansaBioMed Life Sciences] TFF-MV, Large EV isolation
Large EV isolation from cell media, biofluids, plant extracts with minimal loss of small EVs

 

TFF-MV, Separation concentration and purification of large EVs

Ttangential Flow Filtration-based device

 

 

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Tangential flow filtration (TFF) is a rapid and efficient method, usually used for separation and purification of

biomolecules. TFF can be also used to concentrate and desalt sample solutions, and is emerging as a new

technique for EV isolation, if coupled with SEC.

 

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TFF-MV is a filter able to separate large microvesicles (MVs) by size, avoiding the separation by

centrifugation at 10000g, which often causes the loss of part of small EVs. TFF-MV retains vesicles larger

than 150-200 nm, whereas small EVs and circulating molecules pass in the permeate. Retained MVs can be

recovered with a syringe in PBS buffer, without additional purification steps.

 

TFF-MV is a filter cartridge in hollow fibers made of polysulfone having pores of 200 nm size. The filter is

very useful for separating different EVs by size. Indeed, microvesicles bigger than 150 nm are retained inside

the hollow fibers, while small EVs and molecules easily permeate the filter. Microvesicles can be recovered

with a syringe in PBS buffer, without additional purification steps.

 

It works from a mimimal amount of 5 ml of fluids up to liters of fluids.

The filter works with diluted matrices (cell media and urines) and with complex biofluids (plasma, serum

etc.).

 

TFF-MV can be easily washed and it is reusable multiple times.

 

 

Features

• Washalble

• Reusable multiple times

• Easy to use

• Fast concentration of EVs bigger than 150 nm

 

 

Specifications

Sample type;

 

Diluted EV containing matrices (i.e. urine, conditioned cell media)

Filter cartridge;

 

Polysulfone hollow fibres

Filter pore size;

 

200 nm

Filter volume;

 

2 ml

Sample volume per reaction;

 

Recommended sample volume from 10 ml up to several liters if connected

 

 

to mechanical pump.

EV recovery;

 

83%

Concentration time;

 

From 5 minutes

Reusable;

 

Up to 20 times

 

 

How to use TFF Easy

 

 

 

Application

• Separation, concentration and recovery of large EVs (>150 nm).

• Large EV isolation from cell media, biofluids, plant extracts.

• Dialysis and desalting of large EVs.

• Suitable for large EV isolation from 5 ml of fluid.

 

 

TFF-MV: Large EV isolation, with minimal loss of small EVs

 

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EM image and particle size distribution of EVs isolated with TFF-MV

 

 

Currenlty, large MVs are isolated or removed from small EVs by centrifugation (10 000 g for 30 minutes),

which also causes a massive loss of small vesicles. Moreover, different equipment (centrifuges, rotor angle,

etc.) impacts on the final results. TFF-MV allows the removal of MVs, their concentration and purification in

one single step, skipping the centrifugation passage. The isolated MVs result is clean andsuitable for multiple

analyses.

 

 

Product sheet (PDF)

Extracellular Vesicle Isolation Tools_Catalog (PDF)

 

 

Ordering information        

Catalog No.

Product name

Size

HBM-TFF-MV

TFF-MV, Tangential Flow Filter concentrator

kit

 

    

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