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작성일 : 19-05-23
[Biotech Support Group] Albumin & IgG Depletion from Serum/Plasma for Proteomics

 

AlbuVoid™ PLUS - Albumin and IgG Depletion From Serum/Plasma for Proteomics  

 

The classical plasma proteins generally fall into functional categories, forming the vast majority of the mid-

to-high abundance proteome. In serum, these sub-proteomes by mass content are: Albumin 50-60%;

Immunoglobulins 10-20%; Transport Proteins (Transferrin, Apo) 5-10%; Complement related Proteins 3-5%;

Protease Inhibitors 2-5%; and all others 2-5%. While these sub-proteomes are required for normal body

homeostasis,they nevertheless can become dysfunctional during acute-phase and chronic stimuli.

 

So, depending on the needs of the investigation, it can be valuable to consider that one or more of these

categorical sub-proteomes is simply background noise whereby depletion is beneficial. While in other

cases, these same categorical sub-proteomes might provide new data and information and consequently,

should not be depleted. Different AlbuVoid™, AlbuVoid™ PLUS and AlbuSorb™ PLUS workflows support

differentproteomic biases as outlined in the following Table.

 

Products and digest conditions produce different sub-proteome windows of observation. So, depending on

the needs of the investigation, it can be valuable to consider that one or more of these categorical sub-

proteomes is simply background noise whereby depletion is beneficial. While in other cases, these same

categorical sub-proteomes might provide new data and information and consequently, should not be

depleted.Categorically the acute-phase sub-proteomes differentiated in disease may vary greatly from those

associated with chronic sub-proteomes. So there is great benefit in having options to enrich or deplete one

or more of these sub-proteomes.  

 

 Features:

• IgG removal >90% (70-80% of total Immunoglobulins removed)

• Albumin removal >95%

• Seamless and simple < 1 hour protocol

• Low abundance enrichment equivalent to immuno-affinity

• Disposable, cost-effective, no column regeneration or cross-contamination

• Works for most species tested including human, sheep, rat, mouse, bovine

• On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics

• Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays.

 

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BSG's Albumin and IgG Removal Kits offer many such options:

• The 'PLUS' products substantially deplete Immunoglobulins through separations at the protein level.

• The variable regions of Immunoglobulins are extremely heterogeneous, generating a background noise

   across the full LC gradient. On-bead digestion (BASP™) with AlbuVoid™ substantially reduces the

   influence of such Ig peptide features. So in addition to workflow simplicity, BASP™ can be advantageous

   utilized in targeted proteomic workflows whenever the target proteins do not require strong denaturing

   conditions.

• With the exception of Immunoglobulins whereby FASP generates many more spectral features, both strong

   denaturing conditions (FASP) and on-bead digest (BASP™) conditions produce similar protein profiles.

• Both Apolipoproteins and heavily glycosylated proteins (i.e., α1-Acid Glycoprotein) bind poorly to

   AlbuVoid™. For quantitative studies within these classes of proteins, AlbuSorb™ PLUS is recommended.

• The Complement sub-proteome is especially enriched by AlbuVoid™ PLUS. The digest conditions may

   bias towards one or more functional sub-populations, likely due to conformational transitions and protein-

   protein interactions (i.e., Factor Bb, Properdin) that occur upon activation. This needs further investigation.

• The low abundance sub-proteome is enriched 5+ fold with AlbuVoid™ and 4+ fold with AlbuSorb™ PLUS.

 

  

Product Sheet (PDF)

 

 

Ordering informations

    

Catalog No.

Product Name

Size

NP-AVK-05

AlbuVoid™ PLUS Kit

5 preps  5, 25µl Serum samples

NP-AVK-10

10 preps  5, 25µl Serum samples

 

 

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