Cellagen Technology LLC
 
작성일 : 19-04-01
[Cellagen Technology LLC] Mut Express Fast Mutagenesis Kit V2
Mut Express MultiS/II Fast Mutagenesis Kit V2

 

Mut Express MultiS Fast Mutagenesis Kit V2

 

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The Mut Express MultiS Fast Mutagenesis Kit V2 is designed for rapid mutagenesis up to 5 seperate sites

based on ClonExpress rapid cloning technology. The PCR amplified target fragments are treated with DpnI to

remove the template DNA, and then cyclized and transformed into E. coli to complete the site-directed

mutagenesis.

 

The kit is consisted of two modules: the Phanta Max Super-Fidelity DNA Polymerase amplification module

and the ClonExpress rapid cloning module. The ultra-fidelity of Phanta Max DNA polymerase significantly

reduces random mutations during amplification of up to 20 kb in length. The ClonExpress rapid cloning

module allows homologous recombination reactions to replace the conventional annealing ring-forming

reactions, requiring much less template and more flexible primer design strategy. Benefited from the efficient

seamless "ligation" of the PCR products by ClonExpress technology, the kit can generate up to five separate

site mutations in a single mutagenesis reaction. Highly optimized reaction buffer, time-saving operating

procedures and high site-directed mutagenesis efficiency make Mut Express MultiS Fast Mutagenesis Kit V2

the preferred kit for DNA multi-site mutagenesis.

 

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Kit compoents

 

 

C215-01 (10 rxn)

 

C215-02 (25 rxn)

 2x Phanta Max Buffer ;

 

1.25 ml

 

3 x 1.25 ml

 dNTP Mix (10 mM each) ;

 

50 ul

 

125 ul

Phanta Max Super-Fidelity DNA Polymerase ;

 

 50 ul

 

125 ul

 Dpn I (10 U/μl) ;

 

 50 ul

 

125 ul

 5x ClonExpress MultiS Buffer ;

 

40 ul

 

100 ul

 Exnase MultiS ;

 

 20 ul

 

50 ul

* Storage ; Store the kit at -20°C or below.

             

 

Mut Express II Fast Mutagenesis Kit V2

 

The Mut Express II Fast Mutagenesis Kit V2 is designed for rapid site-directed mutagenesis based on

ClonExpress rapid cloning technology. The PCR amplified target fragments are treated with DpnI to remove

the template DNA, and then cyclized and transformed into E. coli to complete the site-directed mutagenesis.

 

The kit consists of two modules: the Phanta Max Super-Fidelity DNA Polymerase amplification module and

the ClonExpress rapid cloning module. The ultra-fidelity of Phanta Max DNA polymerase significantly

reduces random mutations during amplification of up to 20 kb in length. The ClonExpress rapid cloning

module allows homologous recombination reactions to replace the conventional annealing ring-forming

reactions, requiring much less template and more flexible primer design strategy. Benefited from the efficient

seamless "ligation" of the two PCR products by ClonExpress technology, the kit can generate two separate

site mutations in a single mutagenesis reaction. Highly optimized reaction buffer, time-saving operating

procedures and high site-directed mutagenesis efficiency make Mut Express II Fast Mutagenesis Kit V2 the

preferred kit for DNA site mutagenesis.

 

Kit compoents

 

 

C214-01 (10 rxn)

 

C214-02 (25 rxn)

 2x Phanta Max Buffer ;

 

1.25 ml

 

1.25 ml

 dNTP Mix (10 mM each) ;

 

20 ul

 

50 ul

Phanta Max Super-Fidelity DNA Polymerase ;

 

 20 ul

 

50 ul

 Dpn I (10 U/μl) ;

 

 20 ul

 

50 ul

 5x ClonExpress II Buffer ;

 

40 ul

 

100 ul

 Exnase II ;

 

 20 ul

 

50 ul

* Storage ; Store the kit at -20°C or below.

 

 

Ordering informations

    

Catalog No.

Product Name

Size

C215

Mut Express MultiS Fast Mutagenesis Kit V2

10 rxn & 25 rxn

C214

Mut Express II Fast Mutagenesis Kit V2

10 rxn & 25 rxn

 

 

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