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작성일 : 19-03-12
[Invent Biotechnologies, Inc.] Lipid Raft Isolation Kit for Mammalian Cells/Tissues

Minute™ Lipid Raft Isolation Kit for Mammalian Cells/Tissues 

 

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Lipid rafts are small plasma membrane domains containing high level of cholesterol and

sphingolipids.  Lipid rafts have been implicated in numerous cellular processes including but

not limited to signal transduction, membrane trafficking and protein sorting. Lipid-modified

proteins and some transmembrane proteins are concentrated in the rafts while other proteins

are excluded. Lipid rafts are also found to be associated with Na+/K+ ATPase on plasma

membrane. Lipid rafts are traditionally isolated by sucrose-gradient or OptiPrep gradient using

ultracentrifugation that requires large amount of starting material. The protocol is tedious and

time consuming. This kit was developed using our proprietary spin-column-based

technologies, offering a rapid and easy way to isolate lipid rafts. Total membrane fraction of

the sample is first isolated and treated with a non-ionic detergent containing buffer, followed

by isolation of detergent resistant fraction by flotationcentrifugation using just a table top

microcentrifuge. Highly enriched lipid rafts can be obtained from cultured cells/tissues in less

than 90 min without using density gradient and ultracentrifugation.

 

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Ordering information

     

Catalog No

Product Name

Kit components

Amoun

LR-039

Minute™ Lipid Raft Isolation Kit for Mammalian Cells/Tissues

Buffer A

15 ml

Buffer B

10 ml

Buffer C

10 ml

Filter Cartridges

20 units

Collection Tubes (2 ml)

20 units

* For 20 preps

 

 

▣ 관련 페이지 ; Invent Biotechologies, Inc.