Minute™ Total Protein Extraction Kit for muscles
Skeletal and cardiac muscles are highly specialized tissues that are made of well-arranged muscle fibers.
An individual muscle may be made up of hundreds or even thousands of muscle fibers bundled together and
wrapped in a connective tissue covering. The unique organization of the muscle tissue makes it very hard to
homogenize. It is also very difficult to lyse cells in the tissue for total protein extraction.Traditional solution-
based protein extraction methods, such as RIPA, are inefficient and the protein yield is low. The profile of
extracted protein using traditional methods is usually incomplete. This kit provides a highly efficient method
for total protein extraction from human/animal skeletal or cardiac muscle tissues using a combination of
mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers.
The kit features a simple, fast single-tube protocol and optimizedbuffers for muscle tissues. Researchers
have the option to choose either a denaturing or native cell lysis buffer, which is specifically tailored for
muscle tissues. The whole procedure takes less than 10 minutes to complete and the protein yield is in the
range of 2-10 mg/ml. The materials provided are sufficient for 50 extractions.
Comparison of protein extraction efficiency from mouse heart muscles
(RIPA vs SA-06-MS)
Same amount of staring tissue and same volume of lysis buffer (200ul) were used. For RIPA buffer the tissue
was homogenized in a 1.5ml tube with a pestle for 5 minutes and incubated at RT for 10 minutes. The
homogenate was centrifuged at 10,000 x g for 10 minutes and supernatant saved. Same volume of extracted
protein was loaded in 10% SDS-PAGE and stained with Coommassie blue. For SA-06-MS denaturing
bufferwas used. Lane 1. RIPA buffer (R0278, Sigma). Lane 2. SA-06-MS.
Shipping and storage: This kit is shipped and stored at room temperature.