Minute™ High Fidelity ER Isolation Kit for Liver Tissue
Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane
and plasma membrane. Liver is an ER-rich organ and has been used for isolation of ER for decades.
Traditional methods for isolating ER are based on density-gradient ultracentrifugation. The protocol requires a
large amount of starting material and the methods are tedious and time-consuming and contain significant
cross-contamination. Currently, all commercial kits for ER isolation are basedon methods developed in the
1970s. Unlike any other ER isolation kit on the market, this kit employs a patented spin-column-based
technology that is simple and rapid and requires only a small amount of tissue. This kit can differentially
precipitate native ER (mainly rough ER) from frozen liver tissues without using a Dounce homogenizer or
ultracentrifugation. The whole protocol can be done in about 2 hours.
Isolation of ER from frozen mouse tissue. A. Ponceau S stained blot. B. Wester blot of different organelle
markers. PM- Na/K (ATPase, Novus, NB300146S) ER- hCalreticulin (R&D System, MAB38981) GAPDH
(Biolegend, 631401). 1. Total liver lysate, 2 and 3. Isolated ER fraction in duplicate.