Minute™ Total Protein Extraction Kit for Intestines and Mesentery
The intestines are a long and continuous tube that run from stomach to anus. Mesentery is a fold of tissue
that attaches intestines to the body wall. Structurally, intestines and mesentery are very different. However,
from a protein extraction point of view, they have one thing in common: they are very tough and hard to
homogenize. It is also very difficult to lyse cells in the tissues for total protein extraction. Traditional solution-
based protein extraction methods, such as RIPA, are inefficientand the protein yield is very low. The profile of
extracted protein using traditional methods is usually incomplete. This kit provides a highly efficient method
for total protein extraction from human or animal intestines and mesentery tissues using a combination of
mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffers.
The kit features a simple, fast single-tube protocol and optimized buffers for intestine and mesentery tissues.
Researchers have the optionto choose either a denaturing or native cell lysis buffer for specific downstream
applications. The whole procedure takes less than 10 minutes to complete and the protein yield is in the
range of 1-3 mg/ml. The materials provided are sufficient for 50 extractions.
Comparison of protein extraction efficiency from mouse small intestine
(RIPA vs SA-05-IM)
Same amount of staring tissue and same volume of lysis buffer (200ul) were used. For RIPA buffer the tissue
was homogenized in a 1.5ml tube with a pestle for 5 minutes and incubated at RT for 10 minutes. The
homogenate was centrifuged at 10,000 x g for 10 minutes and supernatant saved. Same volume of extracted
protein was loaded in 10% SDS-PAGE and stained with Coommassie blue. For SA-05-IM denaturing
bufferwasused.Lane 1. RIPA buffer (R0278, Sigma). Lane 2. SA-05-IM.
Shipping and storage: This kit is shipped and stored at room temperature.