Minute™ Total Protein Extraction Kit for Skin Tissue
Skin tissue is made up of epidermis, dermis and subcutaneous fat. Because of its unique structure, skin
tissue is notoriously difficult to homogenize. It is also very difficult to lyse the cells in the tissue for total
protein extraction. Traditional solution-based protein extraction methods, such as RIPA, are inefficient and
the yield is very low. The profile of extracted protein is also incomplete with solution-based methods. This kit
provides a highly efficient method for total protein extractionfrom human or animal skin tissues by using a
combination of mechanical extraction and chemical lysis of skin tissues. The kit features a simple and fast
single-tube protocol and optimized buffers for skin tissues. The researchers have the option to choose either
a denaturing or native cell lysis buffer, which is specifically tailored for skin tissue. The whole procedure
takes less than 10 minutes to complete and the protein yield is in the range of 1-5 mg/ml. The materials
provided are sufficient for 50 extractions.
Comparison of protein extraction efficiency from mouse skin
(RIPA vs SA-01-SK)
Same amount of staring tissue and same volume of lysis buffer (200ul) were used. For RIPA buffer the tissue
was homogenized in a 1.5ml tube with a pestle for 5 minutes and incubated at RT for 10 minutes. The
homogenate was centrifuged at 10,000 x g for 10 minutes and supernatant saved. Same volume of extracted
protein was loaded in 10% SDS-PAGE and stained with Coommassie blue. For SA-01-SK denaturing buffer
was used. Lane 1. RIPA buffer (R0278, Sigma). Lane 2. SA-01-SK.
Shipping and storage: This kit is shipped and stored at room temperature.