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작성일 : 18-11-19
[Immagina Biotechnology] Graphene magnetic beads
For separation and purification of basic dyes and aromatic molecules & catalysis

 

Graphene magnetic beads

 

Graphene-doped magnetic beads for separation and purification (MaGO)

For separation and purification of basic dyes and aromatic molecules

 

Immagina MaGO beads have a magnetic core embedded in an agarose structure. Beads are doped with

Graphene Oxide or Reduced Graphene Oxide. MaGO are spherical beads with dimensions of about 50 µm.

The adsorption capacity of MaGO sample was tested using a set of aromatic positively charged dyes (Nile

Blue, Methylene Blue, Rhodamine 6G, and Crystal Violet). The results are reported in Figure 1. All the

molecules tested showed a high value of adsorption capacity and a very fast adsorption kinetics.

 

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Qt (mg/gGO)

t (min)

Nile Blu (NB, H2O)

989

1

Methylene Blu (MB)

622

1

Rhodamine6G (Rh6G)

507

1

Crystal Violet (CV)

685

1

Figure 1. Up: UV-visible spectroscopy of the NB, CV, MB, and Rh6G dyes solutions and the supernatants

after absorption for 1 minute with MaGO Table: Values of absorption capacity (Qt) and the absorption time of

the tested molecules.

 


TetramethylRhodamine molecules (TAMRA) and TAMRA-labelled antibody (IgG-TAMRA) incubated with

MaGO beads for 5-10 seconds followed by magnetic separation of the beads (Figure 2). As revealed from the

Uv-Vis absorption spectra and spectrofluorometric analysis, MaGO beads can efficiently remove the free dye

while the TAMRA-labelled antibody is still in solution (< 10% reduction).

 

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Figure 2. A and B UV Vis of IgG-TAMRA and free TAMRA dye (black lines) incubated with MaGO beads

followed by vortexing for 5 seconds (red lines). C and D. Fluorescence emission of of IgG-TAMRA and free

TAMRA dye (black lines) incubated with MaGO beads followed by vortexing for 5 seconds (red lines).

 


Fast Protocol for free dye/protein separation

• Vortex the MaGO beads tube for 30 seconds

• Put the required MaGO beads volume in a new 1.5 mL tube

• Place the tube on the magnet to separate the beads. Remove the supernatant

• Resuspend the beads in your dye-protein mixture

• Vortex for 5-10 seconds

• Place immediately the tube on the magnet to separate the beads. Remove the supernatant enriched with

  your labelled protein

• Discard the beads containing the free dye

 

 

Palladium decorated Graphene-doped magnetic beads for catalysis (MarGO-Xm)

For catalysis 

 

Video of Methylene Blue reduction with Sodium Borohydride in water catalyzed by MarGO-Xm

 

Immagina MarGO-Xm beads are palladium-decorated graphene magnetic beads. Beads are functionalized

with palladium nanoparticles. MarGO-Xm are spherical beads with dimensions of about 50 µm.

MarGO-Xm particles were tested as catalysts for the catalytic reduction of molecules by sodium boron

hydride. These reactions in the absence of catalysts are very slow and require days to reach completion.

The metallic nanoparticles present on the surface of the graphene can increase the speed of the reaction by

several orders of magnitude. 4-Nitrophenol (4-NP), Congo red (CR), and Methylene Blue (MB) were chosen

as models for testing the catalytic properties of the MarGO-Xm palladium beads. The Turnover Frequency

(TOF) values of the aforementioned reactions are reported in Table 2. The efficiency in degradation of Rh6G,

CR, MB and 4-NP compared with those reported in literature ranks MarGO-Pd among the more active

catalyst.

    

Dy

Reaction time

TOFe

(min.)

(mol DYE*molPd-1*min-1)

4-NP

1.35

30.38

CR

2.000

2.50

MB

0.083

60.24

Table 1: TOF values of MarGO-Xm in different reaction.

 

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Figure 3. UV-Vis spectra of 4-NP reduction before (black) and 1 minute after the addition of the MarGO-Xm

beads (red).

 


Standard Protocol

• Vortex the MarGO-Xm beads tube for 5 seconds

• Put the required MarGO-Xm beads volume in a new 1.5 mL tube

• Place the tube on the magnet to separate the beads. Remove the supernatant

• Resuspend the beads in your reaction mixture

• Vortex for 5 seconds

• Place the tube in a sonication bath and kept it until the reaction is complete, or alternatively mix with a

  thermomixer (do not use magnetic stirrer)

• Place the tube on the magnet to separate the beads. Remove the supernatant to collect the product.

• Discard the beads

 

 

Ordering informations

    

Catalog No.

Product Name

Size

 017-MGC-001

MaGO Graphene-doped magnetic beads

5.0 ml

017-MGA-002

MarGO Palladium decorated Graphene-doped magnetic beads

1.0 ml

 

 

▣ 관련 페이지 ; Immagina BioTechnology S.R.L.