BrainFast Neuronal Maturation Supplement
Medium supplement to accelerate neuronal maturation
BrainXell’s BrainFast Neuronal Maturation Supplement is a proprietary combination of small molecules that is
used to accelerate the differentiation of neural progenitor cells to mature neurons. It is effective with a variety
of neuronal subtypes including, but not limited to, motor neurons, glutamatergic neurons, and GABAergic
BrainFast Neuronal Maturation Supplement will have neural progenitors matured within one to two weeks.
Neuronal progenitors quickly and efficiently become neurons with use of BrainFast . As can be seen in the
example above, virtually no SOX1+ progenitors remain after seven days whereas abundant MAP2+ neurons
are present. The left column shows cells treated with BrainFast, and the right shows control cells.
Rapid neuronal muturation
Neurons rapidly express mature markers within the first week and bigin to display functional activity within
ten days. As can be seen above, both spinal motor and cortical glutamatergic neurons express the pan-
neuronal marker β-III tubulin and the presynaptic marker synapsin.
BrainFast is packaged as two 250 µL vials of 1000X supplement, which is sufficient to treat at least 15 multi-
well plates. With two additions (three for long-term cultures) neurons will begin to mature with high
penetration and minimal cell death.
Poster_Rapid Maturation of Human Neurons (PDF)