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작성일 : 18-08-21
[HansaBioMed Life Sciences] Tangential flow filter for EV concentration

 

TFF-Easy, Tangential flow filter for EV concentration

 

 

Tangential flow filtration (TFF) is a rapid and efficient method, usually used for separation and purification of

biomolecules. TFF can be also used to concentrate and desalt sample solutions, and is emerging as a new

technique for EV isolation, if coupled with SEC.

 

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Our TFF-Easy is a filter cartridge in hollow fibers made of polysulfone, which allows the concentration and

the removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc.), prior

to the EV purification.

 

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The small dimensions of the device allow to concentrate samples from 5 ml up to liters, surmounting the limit

of the TFF technique which is usable for processing big volumes of fluids.

 

 

Features

• Washalble

• Reusable multiple times

• Easy to use

• Fast concentration of EV containing matrices

 

 

Application & Specifications

• Concentration of diluted matrices as cell media or urine prior to EV isolation.

• Easy removal of small molecules and ions from the EV preparation.

• EV dialysis for changing buffer conditions.

• High efficiency of EV isolation if coupled with SEC columns.

• Hollow fiber cut-off 20 nm 

• Filter cartridge made of polysulfone hollow fibers (20 nm pores).

• Minimum volume required 5 ml.

• Sterile.

 

 

Fast and efficient concentration of cell conditioned media or urine

 

LnCAP cell conditioned medium (CCM) has been concentrated in MWCO spin concentrator (100 kDa) and in

TFF-Easy, alternatively. Expression of CD81 has been tested in ELISA assay. TFF-Easy showed better

performance and low variability between different samples.

 

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TFF-Easy allows to change the EV buffer without dialysis process

 

TFF-Easy allows to change the buffer composition without the dialysis process. In the process described in

below, we tested the EV transfer from buffer 1 (PBS1x) to buffer 2 (deionized water*), which present different

conductivity.

The TFF-Easy allows the complete removal of buffer 1, without affecting the EV concentration.

   

Buffer changing process

Conductivity(μS/cm)

Particle concentration(particle/ml)

 EVs in buffer 1 (PBS1x)

15000

4.3 x 1011

 1- Buffer 1 (PBS1x) removal by TFF

15000

 

 2- EV dilution in buffer 2 (water)

240

 

 3- Buffer 1 (PBS1x) residue removal

55

 

 4- EV dilution in buffer 2 (water)

22

 

 EV in buffer 2 (water*)

12

3.58 x 1011

Process for changing EV buffer with TFF-Easy

* EVs are not stable in deionized water. The water as buffer was chosen just to show the difference of

  conductivity between buffer 1 and buffer 2, and the complete removal of buffer 1.

 

 

Product sheet (PDF)

Extracellular Vesicle Isolation Tools_Catalog (PDF)

 

 

Ordering information        

Catalog No.

Product name

Size

HBM-TFF

TFF-Easy, Tangential flow filter for EV concentration

kit

 

    

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