작성일 : 18-08-20
[G-Biosciences] Immobilized Ficin


Immobilized Ficin



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Ficin (or Ficain) is a cysteine protease enzyme (EC isolated from fig latex is that has the

endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason. Ficin is typically used to

cleave mouse IgG1 as this are difficult to cleave with papain and pepsin. In the presence of 1mM or 10mM

cysteine, ficin generates F(ab′)2 and Fab fragments respectively. The Fab and F(ab′)2fragments can be

separated from whole IgG and Fc with either Immobilized Protein A or ion exchange chromatography. 


Immobilized Ficin is a convenient reagent for producing Fab and F(ab′)2 fragments as it avoids the need to

remove the ficin enzyme after digestion




• Generate Fab and F(ab')2 fragments

• For digestion of mouse IgG1

• Eliminates contamination bu Ficin



Important Information

• Activity: 1-1.5mg ficin/ml of resin

• Support: 6% Cross-linked Agarose



Instruction sheet (pdf)



Ordering information   

Catalog No.

Product Name



Immobilized Ficin

5 ml Resin



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