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작성일 : 18-08-20
[G-Biosciences] Immobilized Ficin

 

Immobilized Ficin

 

 

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Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the

endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason. Ficin is typically used to

cleave mouse IgG1 as this are difficult to cleave with papain and pepsin. In the presence of 1mM or 10mM

cysteine, ficin generates F(ab′)2 and Fab fragments respectively. The Fab and F(ab′)2fragments can be

separated from whole IgG and Fc with either Immobilized Protein A or ion exchange chromatography. 

 

Immobilized Ficin is a convenient reagent for producing Fab and F(ab′)2 fragments as it avoids the need to

remove the ficin enzyme after digestion

 

 

Features

• Generate Fab and F(ab')2 fragments

• For digestion of mouse IgG1

• Eliminates contamination bu Ficin

 

 

Important Information

• Activity: 1-1.5mg ficin/ml of resin

• Support: 6% Cross-linked Agarose

 

 

Instruction sheet (pdf)

 

 

Ordering information   

Catalog No.

Product Name

Size

786-793

Immobilized Ficin

5 ml Resin

 

 

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