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작성일 : 18-08-20
[G-Biosciences] Immobilized Papain

 

Immobilized Papain

 

 

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Papain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave

immunoglobulin G molecules in the hinge region.  This cleavage results in the generation of three ~50kDa

fragments; two Fab domains and a Fc domain.  The papain-digested antibody is unable to promote

agglutination, precipitation, opsonization, and lysis. The Fab and Fc fragments can be further separated with

either Immobilized Protein A or ion exchange chromatography. Immobilized Papain is a convenient

reagent for producing Fab and Fc fragments as it avoids the need to remove the papain enzyme after

digestion.

 

 

Features

• Generate Fc and Fab from IgG

• Eliminates contamination with papain enzyme

• Can be used in virtually all scenarios using free papain

 

 

Important Information

• Activity: ≥15‐40 BAEE units/ml resin (One unit will hydrolyze 1.0 μmole of BAEE per minute at pH6.2 at

   25°C)

• Capacity: 250μg papain/ml resin

• Support: 6% Cross‐linked Agarose

 

 

Instruction sheet(pdf)

 

 

Ordering information    

Catalog No.

Product Name

Size

786-790

Immobilized Papain

5ml Resin

 


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