CheminPharma LLC
 
작성일 : 13-05-14
[CheminPharma LLC] Shield-1으로 단백질 발현을 제어할 수 있는 vector
Conditional Inducible Protein Expression Vector (CIPE)
 
Shield-1으로 단백질 발현을 제어할 수 있는 vector 
Conditional Inducible Protein Expression Vector (CIPE)
 
세포 투과성의 저분자 화합물 Shield-1은 단백질 발현의 On / Off를 제어 할 수있는 발현 벡터입니다. in vitro 및 in vivo에서
단백질의 functional analysis에 유용합니다.
 
 
CIPE-100 vector
 
CIPE vectors provide a cloning system where the stability of a specific protein depends on the presence or absence of a cell-
permeable biologically inert molecule Shield. FKBP-derived destabilizing domains (DDs) can typically be fused to any
protein without affecting expression and function. The fusion protein is unstable in the absence of the ligand(Shield-1), and
administration of the Shield-1 causes the fusion protein to be stabilized.
CIPE-100 vector (4620 base pairs) is available with kanamycin resistance for prokaryotic selection and neomycin resistance
for eukayonic resistance.
 
 
 
                                                         CIPE Vector Diagram
 
 
특징
 
• 목적 단백질의 code 영역을 제한효소를 이용하여 cloning 하여, FKBP(FK506-binding protein)의 불안정화영역(Destabilized
  Domain, DD) 융합 단백질을 발현합니다.
• DD 융합 단백질은 proteasome에 의해 분해됩니다. DD 융합 단백질은 Shield-1과 결합해 안정화하여, 세포 내에
  축적합니다 .
• Shield-1 농도 의존적으로 단백질의 발현 수준을 제어 할 수 있습니다.
• DD 영역의 양단에 MCS (Multiple Cloning Site)가 존재하기 때문에, 목적 단백질의 N 말단 또는 C-말단 어느 것에도
  DD 영역을 융합 할 수 있습니다.
• Kanamycin 와 Neomycin 의 선택이 가능합니다.
 
 
Shield-1
 
A POWERFUL TOOL FOR ANALYZING AND CONTROLLING PROTEIN for in VITRO applications.
 
Shield-1 is novel cell permeable small molecule. It a powerful technique to probe biological processes by conditionally
perturbing the function of specific proteins.
This new technique enables you to directly manipulate a specific protein of interest at the in vitro level, making it a
powerful tool for analyzing protein function.
 
 
 
                                     Shield-1의 화학 구조
 
 
Application
 
• Drug Discovery
• Human Therapeutics
• Protein Expression
• Tissue Repair
 
 
 
 
Reference for Shield-1
 
1. “A Directed Approach for Engineering Conditional Protein Stability using Biologically Silent Small Molecules.”
   Maynard-Smith, L. A.; Chen, L.-C.; Banaszynski, L. A.; Ooi, A. G. L.; Wandless, T. J. J. Biol. Chem. 2007, 282, 24866.
2. “Chemical Control of Protein Stability and Function in Living Animals.” Banaszynski, L. A.; Sellmyer, M. A.; Contag, C. H.;
   Wandless, T. J.; Thorne, S. H., Nature Medicine 2008, 14(10), 1123-27.
 
 
Aqua Shield-1
 
WATER SOLUBLE Aqua Shield-1 - A POWERFUL TOOL FOR ANALYZING and controlling PROTEIN *FUNCTION* for in
VIVO applications.
 
 
특징
 
• Superior binding affinity
• Is a many fold improved aqueous solubility
• Can be formulated for oral or i.v. route
• Designed for in vivo applications
 
 
Diagram of Shield -1 of and Aqua Shield -1
 
                                   Stabilization of DD protein by Aqua-Shield 1 and Shield -1
 
 
Application
 
• Drug Discovery
• Human Therapeutics
• Protein Expression
• Tissue Repair
 
 
Ordering informations
 
Catalog No.
Product Name
Size
CIP-S1-kit CIPE-100
 Kit Shield-1 Vector
CIPE vector &  Shield-1
CIP-AS1-kit CIPE-100
Kit Aqua Shield-1 Vector
CIPE vector &  Aqua Shield-1
CIP-S1-00001
Shield-1
0.1mg
CIP-S1-00005
Shield-1
0.5mg
CIP-S1-0001
Shield-1
1.0mg
CIP-S1-0005
Shield-1
5.0mg or 5 x 1.0mg
CIP-S1-0050
Shield-1
50.0mg
CIP-AS1-00001
Aqua Shield-1
0.1mg
CIP-AS1-00005
Aqua Shield-1
0.5mg
CIP-AS1-0001
Aqua Shield-1
1.0mg
CIP-AS1-0005
Aqua Shield-1
5.0mg or 5 x 1.0mg
CIP-AS1-005
Aqua Shield-1
50.0mg
 
 * Bulk 공급도 가능하오니 별도로 문의하여 주십시오.