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작성일 : 18-01-31
[Emfret Analytics] The multipurpose antibody for analysis of GPVI

 

The multipurpose antibody for analysis of GPVI

 


The monoclonal antibody JAQ1 reacts with the platelet collagen receptor GPVI. On the one hand, it inhibits

collagen-induced aggregation of mouse platelets, whereas cross-linking of JAQ1 by a secondary antibody

induces platelet activation and aggregation.

JAQ1 can be used for immunoprecipitation, immunohistochemical analysis of acetone-fixed frozen sections,

immunofluorescent staining, and Western blot analysis under non-reducing condtions.

     

 

JAQ1 recognizes GPVI in Western blot analysis

 

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After separation of mouse platelet lysate by SDSPAGE (non-reducing conditions) and transfer to PVDF

membrane, the membrane was incubated with JAQ1 (1-3 μg/ml) and bound antibody was visualized with

HRP-conjugated anti-rat IgG and ECL.

 

 

Detection of GPVI on mouse platelets

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Mouse blood was diluted 1:20 and 25 μl of this dilution were stained with 5 μl control IgG-FITC (emfret

Analytics, P190-1, black line) or JAQ1-FITC (shaded area) for 15 min at RT and analyzed directly.

Platelets were gated by FSC/SSC characteristics.

 

 

References

1. Clemetson JM, Polgar J, Magnenat E, et al. (1999) The platelet collagen receptor

   glycoprotein VI is a member of the immunoglobulin superfamily closely related to FcalphaR

   and the natural killer receptors. J Biol Chem. 274(41):29019-24

2. Nieswandt B, Watson SP. (2003) Platelet-collagen interaction: is GPVI the central

   receptor? Blood. 102(2):449-61.

3. Nieswandt B, et al. (2000): Expression and function of the mouse collagen receptor

   glycoprotein VI is strictly dependent on its association with the FcRgamma chain. J Biol

   Chem. 275(31):23998-4002

 

 

Ordering information

    

Catalog No.

Product Name

Size

M011-0 (PDF)

The multipurpose antibody for analysis of GPVI

0.5 mg/ml

M011-1 (PDF)

The multipurpose antibody for analysis of GPVI

1.5 ml / 300 tests

 

 

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