M. Tuberculosis ClpP Protease Assay
The disease of tuberculosis (TB) is a serious threat to global public health. It is estimated that infection of
M. tuberculosis (Mtb) causes more than 1 million deaths annually. The protein degradation enzyme ClpP
protease of Mtb is a proven drug target against Mtb. ProFoldin’s high throughput assay for Mtb ClpP
protease is based on cleavage of a labeled peptide substrate that generates fluorescence at 460 nm
(excitation at 380 nm). The assay can be performed in a 384-well or 96-well plate format for tests of Mtb ClpP
protease activities and throughput screening of inhibitors against M. tuberculosis.
M. Tuberculosis ClpP Protease Assay Kit
includes 3100 µl of Assay Buffer and 310 µl of 10 x Substrate and 3500 µl of Stop solution. It is for 100 assays
in 384-well plate format. All assay reagents except the enzyme are included.
M. Tuberculosis ClpP Protease Assay Kit Plus
includes 3000 µl of Assay Buffer, 310 µl of 10 x Substrate, 3500 µl of Stop solution and 31 µl of 100 x Mtb
ClpP1P2 protease. It is for 100 assays in 384-well plate format. All assay reagents are included.