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작성일 : 17-12-22
[Array Bridge] Pichia Host Cell Protein ELISA Kit

 

Pichia Host Cell Protein ELISA Kit

 

Complete kit for the determination of Pichia host cell protein contamination in bulk products expressed in

Pichia expression system

 

 

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The Pichia Host Cell Protein (HCP) ELISA kit is designed to quantitatively measure host cell protein

contamination in bulk products expressed in Pichia expression systems. Please read the complete kit insert

before performing this assay. A series of Pichia protein standards are made from a provided stock solution

and used to generate a standard curve for the assay and all unknown sample concentrations should be read

off this standard curve. Pichia standards or diluted unknown samples are pipetted into the provided 96-well

plate which has been pre-coated with anti-Pichia HCP antibodies to capture Pichia proteins from biologics

samples. Following an incubation to allow capture of the Pichia protein by the antibodies on the plate, a

second anti-Pichia HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the

captured Pichia proteins. After 45 minute incubation, the plate is washed and a Streptavidin-HRP (Horse

Radish Peroxidase) conjugate is added and incubated for 30 minutes. The Streptavidin-HRP conjugate will be

captured by any biotin labeled antibody bound to the plate. Following a wash step to remove unbound

conjugate, TMB substrate is added and is converted by the captured HRP to a colored product in proportion

to the amount of HCP bound to the plate. After a ten minute incubation to allow color development, the

reaction is stopped and the intensity of the generated color is detected in a spectrophotometer plate reader

capable of measuring 450 nm wavelength. A standard curve will be generated from the Pichia protein

standards and used to calculate the concentration of Pichia proteins in the unknown samples, after making

suitable correction for the dilution of the sample.

 

 

Supplied Components

• Coated Clear 96 Well Plates

• Pichia Protein Standards

• 5 x Dilution Buffer

• 10 x PBS-T

• Reporting antibody

• Streptavidin-HRP Conjugate

• TMB Substrate

• Stop Solution

• Plate Sealer

 

 

  Product information (PDF)

 

 

Ordering information   

Catalog No.

Target

Size

AB000113

 Pichia Host Cell Protein ELISA Kit)

1 kit & 5 kits

 

 

▣ 관련 페이지 ; Array Bridge  (바이오시밀러/Biosimilar 구조 평가 ELISA kits, etc.)