Invent Biotechnologies, Inc.
 
작성일 : 17-12-13
[Invent Biotechnologies, Inc.] Hi-efficiency Saliva Exosome Isolation Kit

 

Minute™ Hi-efficiency Saliva Exosome Isolation Kit

 

 

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Diagnoses using exosomes derived from saliva have been attracting great attention in recent years due to the

ease of sample collection. However, saliva samples are challenging when it comes down to exosome

isolation. In addition to cells and cell debris, large amount of amylase, mucin and glycoprotein present in

saliva, making the samples viscous and hard to manipulate. Pre-treatments, such as sonication and dilution,

are often required. In many cases, the salivasamples can still be difficult to handle even after pre-treatments.

This product is specifically designed to address these issues using the proprietary saliva filters. Highly

viscous saliva samples can be converted to non-viscous solution instantly by passing the samples through

the filter. Exosomes can then be readily precipitated from as less as 100 µl saliva using the highly effective

non-PEG-based reagent.

 

 

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Fig.1. SDS-PAGE(10%) of precipitated exosomes from 300 ul saliva. Protein profiles of leading brand in the

market (A) and SE-030 (B).

Fig.2. A, Exosomes precipited from 100 and 300 ul saliva by SE-030 and probed with anti-CD63 in WB. Fig.2

B, comparison of exosomes precipitated from 300 ul saliva by leading brand in the market(1) and SE-030(2).

Isolated exosomes were probed with anti-Alix and anti-CD63.

 


Instruction sheet (PDF)

 

 

Ordering information

     

Catalog No.

Product Name

Kit components

Amount

SE-030

Minute™ Hi-efficiency Saliva Exosome

Isolation Kit

Exosome Precipitation Reagent

15 ml

Filter Cartridge

50 ea

2.0 ml collection tubes

50 ea

* For 50 preps

 

 

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