Minute™ Nuclei and Cytosol Isolation Kit for Adipose Tissues
Adipocytes are the major energy storage sites in the body and they also have critical endocrine functions.
Therefore, understanding the development and function of adipocytes are essential to understanding
metabolic homeostasis under physiological and pathological conditions. Fractionation of cellular components
into nucleus and cytosolic fraction is a common practice in the lab. However, when it comes to adipocytes,
separation of these two fractions are much moredifficult than other tissues because of high concentration of
lipid droplet and low protein content of adipocytes. Methods reported in the literature for isolation of nuclei
and cytosolic fraction from adipose tissues are tedious and time consuming and as much as 50 grams of
tissue are required. This kit provides a very rapid and simple method for obtaining high purity nuclei from
adipose tissues and most important of all, only milligram amounts of tissues are required making it possible
to isolate nuclei andcytosol from small animal and biopsy.
Nuclei isolated from white adipose tissues oh rat, chickens, pig and ship.
The nuclei were stained with trypan blue.
SDS-PAGE and Western blot of rat white adipose proteins.
T, total protein, N, nuclear protein and C, cytosol proteins.