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작성일 : 17-12-11
[AIEGEN Biotech] Probe, target & illuminate only the Cancer Cell Mitochondria
생세포와 고정세포 모두에 사용 할 수 있는 암세포 특이적 형광 프로브입니다.

 

AIE Cancer Yellow, Target and illuminate only the Cancer Cell Mitochondria

생세포와 고정세포 모두에 사용 할 수 있는 암세포 특이적 형광 프로브

 

   

일반적인 형광물질은 ACQ(Aggregation Caused Quenching) 방식으로 응집하면서 발광이 약해지는 문제가 있습니

다. AIEGEN Biotech 사의 probe는 AIE(Aggregation-Induced Emission) 방식으로, 응집되면서 집합체를 형성하면

강한 형광을 발합니다.

 

 

 클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

 

The product can be used for quick cell imaging as well as fixed localized imaging.

The product can serve as a photosensitizer to generate reactive oxygen species (ROS) to induce cell

apoptosis, which can be used for photodynamic therapy.

The product can be excited by 405 nm laser of confocal microscope after co-cultured with cell and the

greenish-yellow signal can be collected above 500 nm.

 

 

Specifications;

• Purpose: Mitochondria staining and induce cell apoptosis

• Color: Orange

• Imaging platform: Fluorescence microscope, Confocal microscope

• Pack size and quantity: 10 μmol

• Detection method: Fluorescence

• Excitation / Emission (nm): 430±20 / 560±50

• Recommended transport condition: Room temperature

• Product declaration: Only used for research. Do not apply to any detection procedure.

 

 

The product has been tested working on

• Human cervix HeLa

• Human breast MDA-MB-231

• Human lung HCC827

• Human breast MCF-7

• Human lung A549

• Human liver HepG2

• Human lung PC-9

 

 

Examples of use;

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Figure 1: Co-localization imaging of HeLa cells stained with MitoTracker Red and AIETM

Cancer Yellow. (a) Bright-field image and (b and c) fluorescent images of HeLa cells stained with (b)

MTR (50 nM) and (c) AIETM Cancer Yellow (200 nM) for 20 min. (d) Merged image of panel (b) and (c).

λex: 540~580 nm (MTR) and 400~440 nm (AIETM Cancer Yellow); scale bar = 20 μm.

 

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Figure 2: Differentiation of cancerous HeLa cells from normal COS-7 cells by AIETM Cancer

Yellow. (a and c) Bright-field and (b and d) fluorescent images of (a and b) HeLa cells and (c and d) COS-

7 cells incubated with 200 nM of AIETM Cancer Yellow for 20 min.

 

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Figure 3: Differentiation of cancer cells from normal cells by AIETM Cancer Yellow. (af)

Fluorescent images of different (ad) cancer cells and (ef) normal cells stained with AIETM Cancer Yellow

(200 nM) for 20 min. λex: 400~440 nm.

 

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Figure 4: Co-culture different combinations of cancer cell and normal cell in culture medium

with AIETM Cancer Yellow. (a, c, e and g) Bright-field images and (b, d, f and h) corresponding

fluorescent images of (a and b) HeLa and COS-7 cells, (c and d) MDA-MB-231 and COS-7 cells, (e and f)

HeLa and MDCK-II cells and (g and h) MCF-7 and COS-7 cells incubated in Dulbecco’s Modified Eagle

Medium (DMEM) with AIETM Cancer Yellow. Light blue rectangles represent cancer cells and yellow circles

represent normal cells. All the images share the same scale bar: 20 μm.

 

 클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Figure 5: AIETM Cancer Yellow selectively kills cancer cells through PDT. (a,b) Change in

mitochondrial morphology before and after white light irradiation. (c) Cell viability of HeLa cells and COS-7

cells stained with different concentrations of AIETM Cancer Yellow in the absence or presence of white light

irradiation.

 

 

Ordering information;     

Catalog No.

Product Name

Size

N/A

AIETM Cancer Yellow

10 μmol

 

 

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