RiboSolutions
 
작성일 : 13-05-06
[RiboSolutions] 높은 활성의 Cyanase Nuclease를 제거하는 Cartridges
Cyanase™ Inactivation Cartridges
 
높은 활성의 Cyanase™  Nuclease를 대용량으로 제거하는 Cartridges
Cyanase™ Inactivation Cartridges
 
 
The Cyanase™ Inactivation Cartridges combine the novel Cyanase™ inactivation resin into easy to use cartridges for fast
and easy removal of Cyanase from high throughput cell lysis and Pre-PCR clean up. No addition of buffer or resin to
reactions, and resin does not react with anything but the Cyanase nuclease *.
(* :  간편한 세포 파쇄장치, Cyanase™ Cell Lysis System)
 
 
                           
 
 
Descriptions
 
New Cyanase™ Inactivation Cartridges combine 50 µL of Inactivation Resin into a 700 µL silica based spin column for Easy
Removal of Cyanase™ from any reaction. Each spin column is a 10-20 µm pore size / polypropylene, snap off base, with
sealable screw cap. Each kit contains 20 Columns pre loaded with enough resin for up to 50 U of Cyanase™ removal.
Reactions are mixed and incubated inside the spin column in the same manner that is used for the standard inactivation
resin. After mixing for 20 minutes, samples are then spun down into a provided collection tube for downstream
processing. No worry of contaminating downstream samples with resin, no volume additions, and no fuss. Just another
way the Cyanase™ System is the new alternative to Benzonase®.
 
Features
 
Ideal For High Throughput Proteomics Applications.
No Addition of Buffer or Resin to Reactions.
Silica Based Columns are Easy to Use.
Can Be Used for Pre PCR Treatment of High Sensitivity Assays. Just Treat Master Mix with Cyanase, Add to Column,
  Incubate with Resin, and Spin Down into Collection Tube. Target and Primers are Added After Treatment and PCR is
  Run as Normal.
 
Applications
 
High Throughput Treatment Via Microfuge or Vacuum Manifold for Downstream Proteomics or Nucleic Acid Dependant
  Applications.
Amplicon and Pre PCR Clean Up of Master Mixes.
Treatment of Samples of Low Volume/Unique Buffer Properties Where Additional Buffer/Products are Problematic.
 
 
Specifications
 
 Average Particle Size
 
90 µm
 Cartridge Specifications
 
Silica-based with 10-20 µm pore size / Polypropylene, snap off base, sealable screw cap
 Maximum Volume
 
700 µL
 Storage Buffer
 
50 mM Tris pH 8.0, 2 mM MgSO4.  Store at +4°C
 
Protocols
 
Cell Lysate Treatment:  After incubation of cell lysate with Cyanase™, clarify the sample by filtration or centrifugation
and remove soluble material.  Remove bottom tab from cartridge and place cartridge in a 1.5 mL microcentrifuge tube.  
Spin the cartridge for 30 seconds in a microcentrifuge at 10,000 x g to remove storage buffer.  Remove cap and add
up to 700 µL of total Cyanase treated cell lysate, or up to 50U of total Cyanase™ treated cell lysate to the cartridge,
whichever is smaller.  Shake, invert or pipette the slurry for 20 minutes at room temperature to allow the beads to
interact with the solution.  Place spin cartridge into provided collection tube and spin for 1 minute in a microcentrifuge at
10,000 x g.  Discard cartridge and place cap on collection tube.  Note: For purification of viral DNA particles, due to the
silica nature of the cartridge, make sure the ion concentration of the lysis solution is not excessive to prevent potential
binding to the matrix.  All conditions for which Cyanase™ is active are appropriate for flowthrough of DNA on the column.  If binding somehow does occur, the DNA can be easily released from the silica in most Tris based buffers at 100 mM NaCl or
lower.
 
Pre-PCR DNA Cleanup: For amplicon and DNA removal, treat up to 700 µL of PCR master mix minus the target and
primers by adding 50 U of Cyanase™ for 30 minutes to 1 hour at 37°C.  Note:  Make sure the PCR mix has at least
2-5 mM of Mg2+ in the mix and below 100 mM NaCl or KCl for effective Cyanase™ activity.  Remove bottom tab from
cartridge and place cartridge in a 1.5 mL microcentrifuge tube.   Spin the cartridge for 30 seconds in a microcentrifuge at
10,000 x g to remove storage buffer. Remove cap and add up to 700 µL of total Cyanase treated PCR master mix or up to
50U of total Cyanase™ treated PCR master mix to the cartridge, whichever is smaller.  Shake, invert or pipette the
slurry for 20 minutes at room temperature to allow the beads to interact with the solution.  Place spin cartridge into
provided collection tube and spin for 1 minute in a microcentrifuge at 10,000 x g.  Discard cartridge and place cap on
collection tube.  Add target and primers and continue with PCR reaction.  Under normal conditions, this method will
remove 99.99% of Cyanase™ activity as measured in the functional testing.  For further Cyanase™ removal, the reaction
can be incubated for up to 1 hour with mixing if heating the master mix before PCR is not desired.  Alternatively, the
sample can be heated at 75°C for 20 minutes or 95°C for 5-10 minutes to remove any residual Cyanase™ activity for
sensitive or low copy number testing depending on the nature of the master mix.       
 
Ordering information ;
 
Catalog No.
Description
Size
1015
Cyanase™ Inactivation Cartridges
20 reactions
 
 
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