CD1d Dextramer, reagent for monitoring NKT cells

CD1d Dextramers are flow cytometry reagents for identification and sorting of Natural Killer T (NKT) cells.

The number and function of NKT cells play an important role in regulating the immune response in many

autoimmune diseases, cancer, graft versus host disease, inflammation and various infectious diseases.

The CD1d/α-GalCer Dextramers displays CD1d molecules loaded with α-GalCer, or without a unique lipid,

unloaded CD1d. Both human and mouse CD1d can stain cells of mouse and human origen, although not

identical NKT cell populations are found between species.

Features;

• Superior separation

• High stability

• Reproducible results

• Quality controled

• Available with FITC, PE and APC.

CD1d/α-GalCer Dextramer:

Human CD1d: Cat. No. XD8002

Mouse CD1d: Cat. No. YD8002

* The CD1d/α-GalCer Dextramer displays CD1d molecules loaded with the glyco-lipid, α-GalCer.

CD1d/unloaded Dextramer:

Human CD1d: Cat. No. XD8001

Mouse CD1d: Cat. No. YD8001

* Load your lipid of interest to generate a unique CD1d/lipid Dextramer. Can be used as negative control

  reagent. The CD1d/unloaded Dextramer reagent displays CD1d molecules without loaded lipid antigen.

Protocol for loading of lipids into CD1d_unloaded Dextramers (PDF)

Human CD1d Dextramer labled with either FITC, PE AND APC

Detection of NKT cells in human PBMCs using CD1d/α-GalCer Dextramers labeled with FITC, PE and APC

fluorochromes, respectively.

Mouse CD1d Dextramer labled with PE

Detection of NKT cells in Mouse CD4 positive splenocytes using mCD1d/α-GalCer Dextramers labeled

with PE.