Icosagen AS
 
작성일 : 13-04-23
[Icosagen AS] E2Tag tagging & detection kit
 
E2Tag tagging & detection kit
 
 
Epitope tagging 기술은 재조합 단백질의 발현, 검출, 동정, 정제를 효율적으로 실시 할 수 있는 시스템입니다. E2 Tag는
bovine papilloma virus 유래의 type 1 전사인자 활성 단백질과 10 아미노산(SSTSSDFRDR)로 이루어진 새로운
epitope입니다.
 
 
pQM-intron kit
 
For E2Tag tagging, expression and detection of recombinant proteins in mammalian cells.
 
Clonning
 
The gene of interest can be cloned into the suitable pQM-intron vector using restriction enzymes indicated in the
   MultiCloning Site (MCS), or through a PCR cloning approach.
The pQM-intron vectors provide E2Tag epitope tagging options which allow for E2Tag epitope tagging at either the
   N- or C-terminus of the protein.
 
Expression
 
The human cytomegalovirus (CMV) immediate early promoter region produces a strong transient expression.
A ß-globin intron located downstream from the enhancer/promoter region can further increase expression of
   recombinant protein.
The translational start sequence used in pQM-E2Tag-N-intron vectors is the Kozak consensus sequence GCCATGG.
The pQM-E2Tag-N-intron vector does not contain a stop-codon.
The pQM-E2Tag-C-intron vector does not contain a translation start sequence.
The polyadenylation sequence provides the signal required for termination of both mammalian transcription and
   translation.
 
Features
 
Easy immunotagging of recombinant proteins.
Eliminates the need to create specific antibodies.
The E2Tag tagged recombinant protein can be detected with E2Tag monoclonal antibody through immunoblotting or
   immunofluorescence analysis.
Anti-E2Tag antibody is visualized with HRP, AP- or FITC-conjugated goat anti-mouse antibodies.
 
Recommendation
 
PCR cloning is recommended if the restriction enzyme sites do not fit with the cloning plan or precise cloning is
   necessary.
For removal of the E2Tag, the protease Pro39 is available (Cat No Y2-100-100). Please note that neither the pQM nor
   pQ vectors contain the Pro39 recognition site. The recognition sequence of Pro39 (FDDVLRLGRAGA/YIFSSDTG)
   can be introduced through the PCR cloning procedure.
 
 
  
 
 
Intrac QP kit
 
For expression and detection of protein-protein interactions in mammalian cells.
 
Clonning
 
The gene of interest can be cloned into the suitable pQM vector using the restriction enzymes indicated in the
   MultiCloning Site (MCS), or through a PCR cloning approach.
The coding sequences of the desired proteins are cloned into the two expression vectors so that each are designated
   individually by the E2Tag or by the E4Tag.
Following co-transfection of the plasmids, cellular expression of the tagged proteins can be observed.
Vectors provide epitope tagging options which allow for E2Tag or E4Tag epitope tagging to take place at either the
   N- or C-terminus of the protein.
 
Expression
 
The human cytomegalovirus (CMV) immediate early promoter region produces a strong transient expression.
The translational start sequences used in the pQM-E2Tag-N and in the pQM-E4Tag-N are the respective Kozak
   consensus sequences GCCATGG and GCCACCATG.
Neither the pQM-E2Tag-N nor the pQM-E4Tag-N vectors contain stop-codons.
Neither the pQM-E2Tag-C nor the pQM-E4Tag-C vectors contain translation start sequences.
The polyadenylation sequence provides the signal required for termination of both mammalian transcription and
   translation.
 
Features
 
Easy immunotagging of recombinant proteins.
Eliminates the need to create specific antibodies.
The anti-E2Tag affinity resin suspension allows for the capture and immunoprecipitation of the protein-protein complex
   of interest.
Visualization of the co-immunoprecipitated protein can be achived with E4Tag antibody or specific antibody through
   immunoblot.
 
 
 
 
IpQM-intron kit  -  Instruction sheet (PDF)
Intrac QP kit  -  Instruction sheet (PDF)
 
 
Ordering information
 
Catalog No.
Product Name
Size
K1-210-001
pQM-intron kit
 -
KI-220-001
Intrac QP kit
-
K1-100-001
pQ kit
-
 
 
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