Calcium and Magnesium assays
Calcium (Ca2+) or Magnesium (Mg2+) is an essential metal ion in biological systems. These divalent ions
are also common components in biochemical buffers and pharmaceutical products. The MicroMolar
Calcium / Magnesium Assay Kit is for measurement of micromolar concentrations of calcium, and
magnesium (10 µM – 120 µM). The assay may also be used to detect other divalent metal ions. The assay
is based on the complex formation of the divalent metal ion with the assay reagent that results in reduction of
the fluorescence intensity (emission 535 nm, excitation 485 nm). Chelators such EDTA and thiol compounds
bind divalent ions and should be avoided in the assay. The assay is compatible with HEPES buffer, low
concentrations of non-ionic detergent (<0.01%), Tris-HCl (<10 mM), and phosphate (< 1 mM). It is not
compatible with thiol compounds such as DTT, 2-mercaptoethanol or cysteine. It is interfered by divalent
metal ions such as Mn2+, Fe2+, Co2+, Ni2+, Cu2+, Hg2+and Zn2+. For specific assays of transitional metal
ions such as Cu2+and Zn2+, please visit http://www.profoldin.com/concentration.html.
The assay kit can be used for measurements calcium or magnesium concentrations in buffer samples or
The MicroMolar Calcium / Magnesium Assay Kit (catalog number DMA200) includes 200 µl of 100 x C56 dye
and 1000 µl of 10 x Reagent E and 50 µl of CaCl2. It is for measurement of 200 samples using 96-well plates.
Cuvettes may also be used for measurements.