Zinc (Zn++) is an essential metal ion in biological systems. Zinc is a cofactor of hundreds of enzymes and
plays important roles in signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and
prostate gland function. Zinc deficiency is associated with malabsorption syndrome, chronic GI, liver
disease, diabetes, renal disease, sickle cell disease, anorexia nervosa, and HIV infection.
NanoMolar Zinc Assay Kit
The NanoMolar Zinc Assay Kit is for measurement of submicromolar concentrations of zinc (100 nM –
1 µM). The assay is based on the principle that binding the fluorescence dye NZA selectively with Zinc
results in increase of the fluorescence intensity (emission 535 nm, excitation 485 nm). The assay is
compatible with regular buffers with different metal ions including 10 mM Mg2+, Ca2+, Cu2+, Mn2+, Al3+,
Fe3+, Ag+ , Co2+ and Ni2+. Chelators such EDTA and thiol compounds bind strongly some of the metal
ions and should be avoided in the assay.
The assay kit can be used for high-throughput measurements of zinc concentrations in chemical and
biochemical reactions, pharmaceutical process or environmental water samples. The kit can also be used
for biochemical assays of enzyme assays associated with zinc metabolism. Each kit provides the reagent
for measurement of 1000 samples using 96-well plates (0.100 ml of sample volume). Cuvettes may also be
used for measurements. The kit also provides a ZnCl2 solution as a control.