Copper (Cu++) is an essential metal ion in biological systems. Many enzymes such as amine oxidase and
galactose oxidase require copper for their biological functions. Low levels of copper are associated with
disorders including mental retardation, depigmentation, anaemia, hypotonia and scorbutic changes in bone.
Levels of copper are key diagnostic indicator of diseases such as Wilson's disease, microcytic hypochromic
anaemia and bone disease.
MicroMolar Copper Assay Kit
The MicroMolar Copper Assay Kit is for measurement of micromolar concentrations of copper (0.01 mM –
0.1 mM). The assay is based on the principle that binding the fluorescence dye MCA selectively with
copper results in decrease of the fluorescence intensity (emission 535 nm, excitation 485 nm). The assay is
compatible with regular buffers with different metal ions including 1 mM Mg2+, Ca2+, Zn2+, Mn2+, and Al3+,
0.1 mM Co2+ and Ni2+. Chelators such EDTA and thiol compounds bind strongly some of the metal ions
and should be avoided in the assay.
The assay kit can be used for high-throughput measurements of copper concentrations in biological
samples or environmental water samples. The kit can also be used for biochemical assays of enzyme
assays associated with copper metabolism.