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작성일 : 17-03-28
[G-Biosciences] CasPASE Apoptosis Colorimetric Assay™

 

CasPASE Apoptosis Colorimetric Assay™

 

 

The CasPASE™ Apoptosis Colorimetric Assay provides a simple and easy to follow method for assaying

caspases 1-10, a key early indicator of apoptosis in mammalian cells.

The assay is based on the detection of cleavage of a synthetic substrate, which is labeled with the

chromophore ρ-nitroaniline (ρNA) at the C-terminal. When liberated from the peptide, ρNA produces an

optical change that can be detected by reading the absorbance at 405nm.

The reaction is selectively and irreversibly inhibited by the peptide Z-VAD-FMK. Comparison of the

absorbance of an induced / apoptotic sample with an uninduced control allows one to determine the fold-

increase in protease activity. The Caspase activity can also be quantitated by using a standard curve

established with the free ρNA dye.

CasPASE™ assays are available for caspase enzymes 1 through 10. Each CasPASE™ assay kit is supplied

with necessary assay buffers, enzyme specific ρNA-substrate, free dye (ρNA), and the potent caspase

inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.

 

Fluorescent Caspase Assays are also available.

 

 

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Features

• Assays for caspase enzymes 1 through 10 are available

• Colorimetric detection (405nm)

• Conveniently adaptable for high-throughout 96-well format

• All kits supplied with enzyme specific (ρNA) & inhibitor Z-VAD-FMK

 

 

Applications

• Monitor apoptosis by measuring various caspase activities in cells and tissues

• Use with purified enzyme preparations, cell extracts, and tissue lysates

• Characterization of caspase enzymes

 

 

Instruction sheet (pdf)

 

 

Ordering informations  


Catalog No.

Product Name

Size

786-858A

 CasPASE™ 1, 4, 5 Assay with Ac-WEHD-pNA Substrate

50 assays

786-858B

 CasPASE™ 1, 4, 5 Assay with Ac-WEHD-pNA Substrate

100 assays

786-859A

 CasPASE™ 2 Assay with Ac-VDVAD-pNA Substrate

50 assays

786-859B

 CasPASE™ 2 Assay with Ac-VDVAD-pNA Substrate

100 assays

786-860A

 CasPASE™ 3, 7, 10 Assay with Ac-DEVD-pNA Substrate

50 assays

786-860B

 CasPASE™ 3, 7, 10 Assay with Ac-DEVD-pNA Substrate

100 assays

786-861A

 CasPASE™ 6 Assay with Ac-VEID-pNA Substrate

50 assays

786-861B

 CasPASE™ 6 Assay with Ac-VEID-pNA Substrate

100 assays

786-862A

 CasPASE™ 8 Assay with Ac-IETD-pNA Substrate

50 assays

786-862B

 CasPASE™ 8 Assay with Ac-IETD-pNA Substrate

100 assays

786-863A

 CasPASE™ 9 Assay with Ac-LEHD-pNA Substrate

50 assays

786-863B

 CasPASE™ 9 Assay with Ac-LEHD-pNA Substrate

100 assays

 

 

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