Kinasera OU
 
작성일 : 17-02-24
[Kinasera OU] ARC(CK2)-Fluo Protein Kinase CK2 assay kit
Protein kinase CK2 저해물질 탐색에 유용한 kit

 

ARC(CK2)-Fluo Protein Kinase CK2 assay kit

For screening/characterization of inhibitors of the protein kinase CK2 저해물질 탐색에 유용한 kit

 

 

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Fig. 1. Workflow

 

 

The ARC(CK2)-Fluo590 Protein Kinase Assay Kit is for a homogenous, high-throughput binding/

displacement assay designed for determination of the concentration of active form of the protein kinase and

screening/characterization of inhibitors of protein kinase CK2 (Specifications).

The principle of the assay relies on the competitive displacement of the fluorescent probe [CK2-FLUO590]

from its complex with the protein kinase by the inhibitor (Fig. 2) . CK2-FLUO590 probe possesses high affinity

towards CK2α (KD = 0.4 nM), it is binding with high affinity to both the free CK2α catalytic subunit and CK2

holoenzyme and enables the characterization of both ATP- and substrate competitive inhibitors due to its

unique bisubstrate character (simultaneous association with both binding sites of the protein kinase).

 

 

Specifications

Application;

 

Determination of Kd values for both ATP- and substrate-competitive protein kinase

 

 

CK2 inhibitors

Targets;

 

CK2 (both the free CK2α catalytic subunit and CK2 holoenzyme)

Detection principle;

 

Fluorescence anisotropy or polarization

 

 

Ex: 540 nm/ Em: 590 nm

Assay setup;

 

Homogeneous, 1-step, mix & read; 384-well low-volume format

Assay volume;

 

10 - 25 μl

Probe;

 

CK2-FLUO590 probe

 

 

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Fig. 2. Principle of ARC(CK2)-Fluo Protein Kinase Assay Kit

 

The fluorescent probe CK2-FLUO590 probe has low molecular weight (MW < 2000) and possesses low value

of fluorescence anisotropy (polarization) in solution. When CK2-FLUO590 probe binds to the protein kinase

CK2, a high molecular weight complex is formed (MW > 40000) which leads to increase of anisotropy of the

solution. An inhibitory compound competes with CK2-FLUO590 for binding to the kinase and displaces the

probe from the complex resulting in decrease of fluorescence anisotropy.

 

 

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Fig. 3. Displacement of the probe CK2-FLUO590 from the complex with CK2α1-335 by

inhibitors or nucleotides ATP and GTP.

 

  

Brochure (PDF)

 

 

Ordering information

    

Catalog No.

Product Name

Size

 CK2-FLUO590

ARC(CK2)-Fluo, Protein Kinase CK2 Assay Kit

kit

 

 

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