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작성일 : 17-02-08
[HansaBioMed Life Sciences] Exo-GREEN, green fluorescent dye
For EV(Extracellular Vesicle) tracking

 

Exo-GREEN, green fluorescent dye

For EV(Extracellular Vesicle) tracking

 

 

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Exo-GREEN kit consists in a buffered lipid dye and a mini size exclusion chromatography (SEC) column.

The fluorecent probe, added to purified vesicles, penetrates into the vesicle membrane, allowing a permanent

and stable fluorescent labeling. Vesicles are subsequently separated by the probe excess through a SEC

mini column. Fluorescent labeled vesicles are stable for long term storage at -20°C (approximately 6 months).

 

 

Features

• Long term stability of labeled EVs stored at -20°C.

• Adapt for labeling exosomes and microvesicles isolated from diff erent matrices.

 

 

Applications

• Fluorescent labeling of pre-isolated exosomes and microvesicles.

• EV traking in cell biology experiments.

 

 

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Exo-GREEN, performance and applications

 

Exo-GREEN stabily labels Exosomes and Microvesicles for fl uorescence microscopy studies 50 μg of

purified exosomes from BLCL21 cell media were treated with Exo-GREEN, following the manufacture’s

instructions. Labeled Exosomes were subsequently analyzed by fl uorescence microscopy performed with

Zeiss LSM Dou 510 META(Fig 1). FACS analysis was additionally performed, comparing Exo-GREEN labeled

exosomes vs unlabeled and with purified exosomes stained by FACS using CD9 antibody (Alexa 488) (Fig2).

Aliquotes of labeled exosomes were stored at -80° and the fluorescence was tested aft er 6 months.

 

 

  Fresh purified Exosomes Exo-GREEN labeled

 

Exosomes Exo-GREEN labeled stored 6 months at -80°C

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Fig. 1. Fluorescent microscopy of Exo-Green labeled exosomes

 

 

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Fig. 2. FACS analysis of purifi ed BLCL exosomes, labeled with CD9-Alexa488, with Exo-GREEN and

unlabeled

 

 

     

Ordering informations

 

Catalog No.

Product Name

Size

HBM-EGREEN-5

Exo-GREEN, green fluorescent dye for EV tracking

5 reactions

HBM-EGREEN-10

10 reactions

 

 

 

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