HansaBioMed OU
 
작성일 : 16-11-15
[HansaBioMed OU] Branched Chain Amino Acid (Leu/Ile/Val) Assay kit

 

Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay kit

Sensitive, Colorimetric Assay

 

 

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The branched-chain amino acids or BCAA’s, refer to the amino acids with non-linear aliphatic side-chains,

namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of

skeletal muscle in the human body. BCAA’s are currently used clinically to aid in the recovery of burn

victims, as well as for strength supplementation for athletes. BCAA’s, primarily Leu, can stimulate insulin

secretion. The BCAA’s have also been implicated in a wide range of other physiological effects.

HansaBioMed's BCAA Assay Kit provides a simple convenient means of measuring the BCAA’s in a variety

of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing

NADH which reduces the probe, generating a colored product (λmax = 450 nm). The BCAA kit measures

BCAA’s in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample).

BCAA’s are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).

 

 

Features

• Simple procedure; takes less than ~40 minutes

• Fast and convenient

 

 

Application & Specifications

• HansaBioMed’s BCAA kit measures BCAA’s in the range of 0 to 10 nmol per sample with a detection limit of

  ~0.2 nmol (~10 µM BCAA in sample).

• Detection method- Absorbance (450 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other

  biological fluids

• Species reactivity- Mammalian

 

 

Kit Components

• BCAA Assay Buffer

BCAA Enzyme Mix

• WST Substrate Mix

• Leu Standard (1 µmol)

 

 

- Storage Conditions: -20°C

- Shipping Conditions: gel pack

- Usage: For research use only.  Not for use in humans

 

 

Ordering information     

Catalog No.

Product name

Size

HBM-K564-100

Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay kit

100 reactions

 

 

 

HansaBioMed 2016 catalog (PDF)    

 

  

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