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작성일 : 16-11-15
[HansaBioMed Life Sciences] N'-Nicotinamide Methyltransferase(NNMT) Inhibitor Screening Assay kit

 

N'-Nicotinamide Methyltransferase(NNMT) Inhibitor Screening Assay kit

The FIRST and ONLY kit on the market for Nicotinamide N-Methyltransferase(NNMT) Inhibitor Screening

 

 

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N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide,

pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production

of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is

expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and

diabetes, indicating it is a potential molecular target for therapy. The NNMT inhibitor screening kit utilizes

SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide

generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH

hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe

generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of

an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a

simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.

 

 

Features

• Rapid assay and simple protocol

• Reliable test for screening potential inhibitors of NNMT

Includes Inhibitor Control, 1-Methylnicotinamide (MNA)

 

 

Application & Specifications

• Screening/studying/characterizing potential inhibitors of NNMT

• Detection method- Fluorescence (Ex/Em = 392/482 nm)

• Species reactivity- Mammalian

 

 

Kit Components

• NNMT Assay Buffer

• NNMT Enzyme

• S-Adenosylmethionine (SAM)

• Nicotinamide

• Enzyme-I

• Enzyme-II

• 1-Methylnicotinamide (MNA) (150 mM)

• Thiol Detecting Probe (DMSO)

• SAM Reconstitution Buffer

 

 

- Storage Conditions: -20°C

- Shipping Conditions: gel pack

- Usage: For research use only.  Not for use in humans

 

 

Ordering information     

Catalog No.

Product name

Size

HBM-K822-100

Nicotinamide Methyltransferase(NNMR) Inhibitor Screening Assay kit

100 reactions

 

 

 

HansaBioMed 2016 catalog (PDF)    

 

  

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