HansaBioMed OU
작성일 : 16-11-15
[HansaBioMed OU] N'-Nicotinamide Methyltransferase(NNMT) Inhibitor Screening Assay kit


N'-Nicotinamide Methyltransferase(NNMT) Inhibitor Screening Assay kit

The FIRST and ONLY kit on the market for Nicotinamide N-Methyltransferase(NNMT) Inhibitor Screening



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N′-Nicotinamide Methyltransferase (NNMT) (E.C. catalyzes the N-methylation of nicotinamide,

pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production

of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is

expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and

diabetes, indicating it is a potential molecular target for therapy. The NNMT inhibitor screening kit utilizes

SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide

generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH

hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe

generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of

an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a

simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.




• Rapid assay and simple protocol

• Reliable test for screening potential inhibitors of NNMT

Includes Inhibitor Control, 1-Methylnicotinamide (MNA)



Application & Specifications

• Screening/studying/characterizing potential inhibitors of NNMT

• Detection method- Fluorescence (Ex/Em = 392/482 nm)

• Species reactivity- Mammalian



Kit Components

• NNMT Assay Buffer

• NNMT Enzyme

• S-Adenosylmethionine (SAM)

• Nicotinamide

• Enzyme-I

• Enzyme-II

• 1-Methylnicotinamide (MNA) (150 mM)

• Thiol Detecting Probe (DMSO)

• SAM Reconstitution Buffer



- Storage Conditions: -20°C

- Shipping Conditions: gel pack

- Usage: For research use only.  Not for use in humans



Ordering information     

Catalog No.

Product name



Nicotinamide Methyltransferase(NNMR) Inhibitor Screening Assay kit

100 reactions




HansaBioMed 2016 catalog (PDF)    



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