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작성일 : 16-11-15
[HansaBioMed Life Sciences] Beta-Secretase Activity Fluorometric Assay kit

 

Beta-Secretase Activity Fluorometric Assay kit

High Sensitive Assay

 

 

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β-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of

Alzheimer’s disease. The β-Secretase activity Assay Kit provides a convenient fluorescence method for

detecting β-secretase activity in biological and purified samples. The assay utilizes a secretase-specific

peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent

emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the

peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent

signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence

intensity.

 

 

Features

• Simple procedure, takes ~ 1 hour

• Fast and convenient

• The assay is sensitive, stable and high-throughput adaptable.

 

 

Application & Specifications

• Convenient fluorescence method for detecting β-secretase activity in biological and purified samples

• Detection method- Fluorescence (Ex/Em 335-355/ 495-510 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other

  biological fluids, etc.

• Species reactivity- Mammalian

 

 

Kit Components

• β-Secretase Extraction Buffer

β-Secretase Reaction Buffer (2X)

• β-Secretase Substrate (in DMSO)

Active β-Secretase (Lyophilized)

• β-Secretase Inhibitor (in DMSO)

 

 

- Storage Conditions: -20°C

- Shipping Conditions: gel pack

- Usage: For research use only.  Not for use in humans

 

 

Ordering information     

Catalog No.

Product name

Size

HBM-K360-100

Beta-Secretase Activity Fluorometric Assay kit

100 reactions

 

 

 

HansaBioMed 2016 catalog (PDF)    

 

  

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