HansaBioMed Life Sciences
 
작성일 : 16-11-15
[HansaBioMed Life Sciences] β-Hydroxybutyrate (β-HB) Colorimetric Assay kit

 

β-Hydroxybutyrate (β-HB) Colorimetric Assay kit

Sensitive, Colorimetric Assay

 

 

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Diabetic ketoacidosis occurs when circulating insulin levels drop to very low levels, shutting off the supply of

glucose to the body. The physiological response is for the liver to produce ketone bodies (acetoacetate,

acetone, and primarily β-hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very

high rate of ketone body production outstrips the body’s ability to utilize them as an energy source and the

blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.

HansaBioMed’s β-HB Assay kit utilizes β-HB Dehydrogenase to generate a product which reacts with our

colorimetric probe with an absorbance band at 450 nm. The kit is an easy and accurate assay to measure β-

HB levels in biological samples. The assay is linear for 1-20 nmol β-HB in up to 100 µl samples or 0.01-0.2

mM of β-HB samples.

 

 

Features

• Simple procedure, takes ~ 40 minutes

• Fast and convenient

• The kit provides an accurate assay for measuring β-Hydroxybutyrate (β-HB) in various sample.

 

 

Application & Specifications

• The assay is linear for 1-20 nmol β-HB in up to 100 µl samples or 0.01-0.2 mM of β-HB samples

• Detection method- Absorbance (450 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other

  biological fluids, etc.

• Species reactivity- Mammalian

 

 

Kit Components

β-HB Assay Buffer

• β-HB Enzyme Mix

β-HB Substrate Mix

• β-HB Standard (1.0 µmol)

 

 

- Storage Conditions: -20°C

- Shipping Conditions: gel pack

- Usage: For research use only.  Not for use in humans

 

 

Ordering information     

Catalog No.

Product name

Size

HBM-K632-100

β-Hydroxybutyrate (β-HB) Colorimetric Assay kit

100 reactions

 

 

 

HansaBioMed 2016 catalog (PDF)    

 

  

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