HansaBioMed Life Sciences
 
작성일 : 16-11-15
[HansaBioMed Life Sciences] Aspartate Aminotransferase (AST or SGOT) Assay kit

 

Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay kit

Sensitive Assay, HTS

 

 

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Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a

transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly

included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may

also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction,

also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal

diseases and trauma. It catalyzes the reaction.

 

Aspartate + α-ketoglutarate ⇔ Oxaloacetate + Glutamate.

 

Diagnostically, it is almost always measured in units/liter (U/L). In AST Assay Kit, an amino group is

transferred of from aspartate to α-ketoglutarate. The products of this reversible transamination reaction are

oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly

colorless probe to color (λmax = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable

as a high throughput activity assay of AST with a detection limit of 10 mU per well.

 

 

Features

• Simple procedure

• Fast, reliable and convenient

• Sensitive assays for measuring AST/ SGOT activity in various biological samples and the kit is also

  suitable for high throughput analyses.

 

 

Application & Specifications

• This assay detects AST/ SGOT with a detection limit of 10 mU per well.

• Detection method- Absorbance (450 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other

  biological fluids, etc.

• Species reactivity- Mammalian

 

 

Kit Components

• AST Assay Buffer

• AST Enzyme Mix

• Developer

• AST Substrate

• Glutamate Standard (0.1M)

• AST positive control

 

 

- Storage Conditions: -20°C

- Shipping Conditions: gel pack

- Usage: For research use only.  Not for use in humans

 

 

Ordering information     

Catalog No.

Product name

Size

HBM-K753-100

Aspartate Aminotransferase (AST or SGOT) Assay kit

100 reactions

 

 

 

HansaBioMed 2016 catalog (PDF)    

 

  

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