HansaBioMed OU
 
작성일 : 16-11-15
[HansaBioMed OU] Glutathione Reductase Activity Colorimetric Assay kit

 

Glutathione Reductase Activity Colorimetric Assay kit

Highly Sensitive Assay, HTS

 

 

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Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione

(GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains

adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.

HansaBioMed’s Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-

readycolorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to

GSH, which reacts with 5, 5′-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax =

405 nm). The assay can detect 0.1 – 40 mU/ml GR in various samples.

 

 

Features

• Simple procedure; takes ~ 30-40 minutes

• Fast and convenient

 

 

Application & Specifications

• The assay can detect 0.1 – 40 mU/ml GR in various samples.

• Detection method- Absorbance (405 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, erythrocyte lysates, as well

  as many other biological fluids etc.

• Species reactivity- Mammalian

 

 

Kit Components

• GR Assay Buffer

• 3% H₂O₂

• Catalase

• TNB Standard (5 mM)

• DTNB

• NADPH-GNERAT™

• GSSG

• GR Positive Control (10 mU)

 

 

- Storage Conditions: -20°C

- Shipping Conditions: gel pack

- Usage: For research use only.  Not for use in humans

 

 

Ordering information     

Catalog No.

Product name

Size

HBM-K761-200

Glutathione Reductase Activity Colorimetric Assay Kit

200 reactions

 

 

 

HansaBioMed 2016 catalog (PDF)    

 

  

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