360-HCP(Host Cell Protein) ELISA 키트는 기존의 HCP 분석에서 문제가 되고 있던, 샘플 중 HCP가 완전히
검출되지 않은 단점을 보완한 최신의 제품입니다.
기존의 generic HCP 분석은 모든 과정을 단 한 종류의 항체로 하지만, "Enhanced generic 360-HCP ELISA"에서는
4 종류의 키트 (각각 다른 anti-HCP 항체를 사용)를 제공합니다. 여러 항체를 사용하여서 샘플에 가장 적합한 항체
를 찾을 수 있습니다.
The 360-HCP (Host Cell Protein) ELISA
The enhanced generic 360-HCP approach produces host cell protein (HCP) assays that are demonstrably
superior to traditional generic HCP assays minimizing well-known limitations including the possibility that
anti-HCP antibodies might not comprehensively detect HCPs present in an end-user's sample. While
traditional generic HCP assays provide just a single antibody that must work for all processes the enhanced
generic 360-HCP ELISA provides four kits, each using a different anti-HCP antibody so you can try multiple
antibodies to find the one that works best for your samples.
This unique approach increases the assay specificity and sensitivity and allows sponsors and biotech
companies to postpone the development of a cost-intensive specific HCP assay until a more informed
decision on the success of a biologic in development can be made.
How it works
How we made the antibodies
Polyclonal HCP antiserum was generated by immunizing rabbits and goats with HCP derived from mock
transfected CHO-K1 and CHO-S cells combined with a unique approach for antigen and antibody preparation
as well as an optimized purification strategy. We used differently prepared antigens: total HCP or fractionated
HCP. Each HCP fraction was used individually for immunization resulting in four differentHCP ELISA kits
(type A to D) that together build up the enhanced generic CHO|360-HCP assay.
Superior HCP Recovery
The CHO|360-HCP ELISA has been widely tested on the basis of a great number of mock CHO-HCP
samples. In most cases, the best recovery was estimated with one of our four generic CHO-HCP assays
(type A to D). In the case of sampel 4(x), a recovery of >90% was estimated using assay type D. Recovery
with assay types A to C was much lower, and recovery with the commercial assay was only 20%.
Sensitivity CHO|360-HCP ELISA Kits (A–D)
• LOD between 0.5 – 1.0 ng/mL
• LOQ between 2 – 3 ng/mL
• Working range 2 – 100 ng/mL
Instruction sheet (PDF)
Qualification report (PDF)