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작성일 : 16-08-10
[HansaBioMed Life Sciences] GAPDH Activity Assay kit
Oxidative stress

 

GAPDH Activity Assay kit

 

 

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GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of

Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in

glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron

metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive.

Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate

quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology.

GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various

samples. In this assay, GAPDH catalyzes conversion of GAP into BPG and an intermediate, which reacts

with a developer to form a colored product that absorbs maximally at 450 nm. Our high-throughput adaptable

assay can detect GAPDH activity as low as 100 µU/mL in a variety of samples.

 

 

Features

• Simple, rapid & convenient

• Can measure GAPDH activity as low as 100 µU/mL in a variety of samples.

 

 

Applications & specifications

• Measurement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity in various tissues/cells

- Analysis of glycolysis and pentose phosphate pathways.

• Detection method - Absorbance (450 nm)

• Sample Type:

- Animal tissues: Liver, Heart etc.

- Cell culture: Adherent or suspension cells.

 

 

Kit Components

• GAPDH Assay Buffer

• GAPDH Substrate

• GAPDH Developer

• NADH Standard (500 nmol)

• GAPDH Positive Control

 

 

- Storage Conditions: -20°C

- Shipping Conditions: gel pack

- Usage: For research use only.  Not for use in humans

 

 

Ordering information     

Catalog No.

Product name

Size

HBM-K680-100

GAPDH Activity Assay kit

100 reactions

 

 

 

HansaBioMed 2016 catalog (PDF)    

 

  

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