Bioenno Tech, LLC
 
작성일 : 16-08-03
[Bioenno Tech, LLC] Fast staining neurons, excellent for fresh tissues

 

Fast staining neurons, excellent for fresh tissues

 

 

The eliteGolgi kit is ideal for staining neurons in the olfactory bulb, cortex, cerebellum, brain stem, and spinal

cord. The kit supplements and extends the capability of our proven superGolgi Kit. This kit can be used in

combination withthe superGolgi Kit and sliceGolgi Kitto achieve a more comprehensive analysis of select

areas of the brain.

 

 

Features

• Reliable and high-contrast staining of cells in the superficial regions of the brain

• Suitable for freshly harvested brain tissues (Not for 4% PFA-fixed, frozen tissues)

• Impregnation time of only 3-6 days

• Streamlined staining protocol

• Sufficient for up to 50 mouse brains

• For in-vitro lab use

• Warranty: 12 months

 

 

The eliteGolgi Kit has been rigorously tested and validated on brain tissues freshly harvested from rats and

mice (see Proven Results below). This kit allows neurons to be impregnated rapidly requiring only 3-6 days

depending on the age, size, and region of the tissues. Moreover, the staining of neurons can be performed

on either free-floating or mounted sections (50~250 µm thickness). The kit can be storedin a dark area at

room temperature (4 to 25 deg C) for up to 12 months.

 

 

Important! The eliteGolgi Kit does not work well on frozen tissues.

 

 

Proven results

In a number of investigations, neurons and dendritic spines have been reliably stained with the eliteGolgi Kit.

The following shows some representative images.

 

 

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Fig. 1. Golgi impregnated and stained neurons in the neocortex

 

The eliteGolgi Kit was used to impregnate and stain the neurons in the neocortex of a postnatal day 18

Sprague Dawley rat. The boxed area in the left panel (taken at 20x objective lens) was magnified (63x) to

show the dendrites, axon, and soma of a stained neuron.

 

 

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Fig. 2. Impregnated and stained cells in the olfactory bulb and spinal cord

 

The stained cells in the olfactory bulb (left panel, taken at 10x objective) and the spinal cord (right, 4x

objective) of a postnatal day 11 rat. Freshly harvested tissue blocks were subjected to Golgi staining via the

eliteGolgi Kit. The impregnation time was 4 days at room temperature (22 ± 1 degree C). The sections were

counterstained with Bioenno Cresyl Violet (Cat # 003003).

 

 

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Fig. 3. Impregnated and stained cells in the olfactory bulb (sagittal section)

 

The eliteGolgi Kit was used to impregnate and stain the cells in the olfactory bulb of a 12-day-old Sprague

Dawley rat. The boxed area in the left panel was magnified (63x) to show the branches of a stained cell.

 

 

 클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Fig. 4. Golgi impregnated and stained cells in the spinal cord (wet section)

 

Golgi staining was performed on saline-perfused spinal cord tissue block (12-day-old rat) using the

eliteGolgi Kit. The impregnation time was 4 days (22 ± 1 degree C). These images were taken from a wet

section (not dehydrated in ethanol, not cleared in xylene or xylene substitute) using 2x, 10x, or 20x objective

lenses. The boxed areas were magnified (10x or 20x objective) to show cells in the posteriorhorn of the

spinal cord.

 

 

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Fig. 5. Golgi impregnated and stained Purkinje cells in the cerebellum (wet section)

 

Golgi staining was performed on freshly harvested cerebellum (not PFA-fixed) of a 12-month-old C57 mouse

using the eliteGolgi Kit. The impregnation time was 4 days (22 ± 1 degree C). The image was taken from a

wet section (not dehydrated & not cleared) using a 20x objective lens.

 

 

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Fig. 6. Golgi-stained dendritic branches of Purkinje cells

 

The eliteGolgi Kit was used to impregnate and stain the dendritic branches of Purkinje cells in the cerebellum

of a 6-month-old C57 mouse. The impregnation took 4 days only. The staining and clarity took 3 min and ~1

min, respectively. The section was air-dried, cleared in xylene, and cover slipped with Permount® mounting

medium.

 

 

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Fig. 7. Neurons in the anterior olfactory nucleus

 

The eliteGolgi Kit was used to impregnate and stain the neurons located in the anterior olfactory nucleus

(lateral) (left panel, 4x objective) of a 12-day-old rat. The boxed area was magnified (20x) to show a stained

neuron and further magnified (63x) to show the dendritic spines.

 

 

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Fig. 8. Neuron in the frontal cortex

 

The eliteGolgi Kit was used to impregnate and stain cells in the frontal cortex (left panel, 4x objective). The

framed area was magnified (20x) in the right to show the branches and dendritic spines of a pyramidal

neuron located in layer III.

 

 

 클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Fig. 9. Two neurons in the somatosensory cortex

 

The eliteGolgi Kit was used to impregnate (4 days, 22 ± 1 degree C) and stain (3 minutes, 22 ± 1 degree C)

neurons in the frontoparietal cortex (somatosensory area). The images were taken from the cortex of a 12-

day old rat using a 20x objective lens.

 

 

Instruction sheet (pdf)

 

 

Ordering information

Catalog No.

Product Name

Size

006690

eliteGolgi Kit

kit