Genetically modified cell lines

 

 

Celther offers genetically modified lines for use in areas such as basic research, target validation, drug

discovery and drug development. Celther modified cell lines give you the tools to take your research to new

heights.

 

 

Mouse immortalized peritoneal mesothelium (MDM)

The MDM cell line is established from mouse peritoneal mesothelium immortalized with large antigen SV40.

The MDM cell line is characterized by a high proliferation rate. The MDM cells are large and planar rapidly

covering the plate surface. At higher confluence, the MDM cells may be efficiently used as a feeder layer.

  

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Organism:

 

Mouse

Tissue:

 

Mesothelium

Disease:

 

Normal

Growth Properties:

 

Adherent

Biosafety Level:

 

1

Product Format:

 

Frozen 1 ml, 1 x 106 cells/vial

   

 

CLTH/EGFRvIII

CLTH/EGFRvIII cell line stably expresses EGFRvIII and neomicin-resistant genes. Both EGFRvIII and

neomicin-resistant genes are under CMV promoter control, allowing high levels of protein expression.

    

Organism:

 

Homo sapiens, human

Tissue:

 

Human embryonic kidney

Morphology:

 

Epithelial

Growth Properties:

 

Adherent

Biosafety Level:

 

1

Product Format:

 

Frozen 1 ml, 1 x 106 cells/vial

    

 

U87MG vIII 4.12

The U87MG vIII 4.12 clone cell line is a permanent line established from cell line derived from malignant

glioma, classified as glioblastoma. U87MG vIII (4.12) cell line stably expresses high level of EGF mutant

receptor vIII and G418-resistant genes introduced by plasmid stable transfection.

   

Organism:

 

Homo sapiens, human

Tissue:

 

Brain

Disease:

 

Glioblastoma; astrocytoma

Morphology:

 

Epithelial

Growth Properties:

 

Adherent

Biosafety Level:

 

1

Product Format:

 

Frozen 1 ml, 1 x 106 cells/vial

 

 

T96/EGFRvIII

The T96 cell line is established from glioblastoma multiforme. The T96/EGFRvIII with additional point mutation

C311Y cell line stably expresses mutated EGFRvIII and neomicin-resistant genes. Both mutated EGFRvIII and

neomicin-resistant genes are under CMV promoter control, allowing high levels of protein expression.

    

Organism:

 

Homo sapiens, human

Tissue:

 

Brain

Disease:

 

Glioblastoma

Growth Properties:

 

Adherent

Biosafety Level:

 

1

Product Format:

 

Frozen 1 ml, 1 x 106 cells/vial

 

 

DKMG/EGFR vIII

Application of the DKMG/EGFRvIII cell line opens new perspectives in EGFRvIII research. EGFRvIII

expression was reinduced in cells, which expressed EGFRvIII in vivo, but due to unknown reasons did not in

vitro. DKMG cells show mutation at DNA level, which is responsible for the expression of EGFRvIII mRNA and

protein, however, in most of these cells the expression of mutatant EGFR gene copies is silenced (these

cells show the amplification of this gene). The performed biotechnological modification results in the EGFRvIII

expression in all cells of the DKMG cell line.

    

Organism:

 

Homo sapiens, human

Tissue:

 

Brain

Disease:

 

Glioblastoma

Growth Properties:

 

Adherent

Biosafety Level:

 

1

Product Format:

 

Frozen 1 ml, 1 x 106 cells/vial

  

 

CLTH/EFGR

The CLTH/EGFR WT were stably transfected with genomic integration of a plasmid coding for EGFR receptor

protein and are expressing Wild Type (WT). The expression of EGFR was confirmed by Western Blotting.

The EGFR WT cells are resistant to antibiotics Puromycin used as a selection marker.

    

Organism:

 

Homo sapiens, human

Tissue:

 

Human embryonic kidney

Morphology:

 

Epithelial

Growth Properties:

 

Adherent

Biosafety Level:

 

1

Product Format:

 

Frozen 1 ml, 1 x 106 cells/vial

  

 

CLTH EGFR / EGFRvIII

The CLTH EGFR WT/ EGFRvIII were stably transfected with genomic integration of a plasmid coding for

EGFRvIII receptor protein and are expressing both Wild Type (WT) and mutated (vIII) form of Epidermal

Growth Factor Receptor (EGFR). The expression of EGFR was confirmed by Western Blotting. The EGFR WT

EGFRvIII cells are resistant to antibiotics Puromycin and G418 used as selection markers.

    

Organism:

 

Homo sapiens, human

Tissue:

 

Human embryonic kidney

Morphology:

 

Epithelial

Growth Properties:

 

Adherent

Biosafety Level:

 

1

Product Format:

 

Frozen 1 ml, 1 x 106 cells/vial

  

 

Pericytes

Pericytes are mesenchymal-like cells that are associated with a network of small blood vessels. Embedded

within the vascular basement membrane and adjacent to endothelial cells, pericytes are involved in

stabilization of the walls of blood vessels, regulation of blood flow and angiogenesis.

These cells not only participate in maturation of endothelial cells, development of new blood vessels but also

help sustain the blood–brain barrier.

Due to their multipotency, pericytes have the ability to differentiate into fibroblasts, smooth muscle cells

adipocytes, osteoblasts, and phagocytes (macrophages)Pericyte markers: CD146, NG2, PECAM, Alpha-

smooth muscle actin (ASMA), Desmin. Celther Pericytes are isolated form brain.

    

Organism:

 

Homo sapiens, human

Product Format:

 

Frozen 1 ml, 1 x 106 cells/vial

  

 

Ordering informations   

Catalog No.

Product Name

Size

CL 01002-CLTH

Mouse immortalized peritoneal mesothelium (MDM)

Frozen 1 ml

CL 01001-CLTH

CLTH/EGFRvIII

Frozen 1 mll

CL 01004-CLTH

U87MG vIII 4.12

Frozen 1 ml

CL 01007-CLTH

T96/EGFRvIII

Frozen 1 ml

CL 01008-CLTH

DKMG/EGFR vIII

Frozen 1 ml

CL 01010-CLTH

CLTH/EFGR

Frozen 1 ml

CL 01011-CLTH

CLTH EGFR / EGFRvIII

Frozen 1 ml

 CL05008-CLTH

Pericytes

Frozen 1 ml