RiboSolutions
 
작성일 : 13-04-13
[RiboSolutions] 간편한 세포 파쇄장치, Cyanase™ Cell Lysis System
 
간편한 세포 파쇄장치, Cyanase™ Cell Lysis System
New Cyanase Lysis kit for cell counts up to 1X108 and Cyanase Lysis kit HL for cell counts up to 1X1011.
 
 
                           
 
 
Features:
 
운반이 용이하고, 저렴한 일회용 세포 파쇄 장치입니다.
조작이 간단하고 기존의 초음파 파쇄법(fracturing method), homogenization, 화학적 용해법보다 빠릅니다.
Complete Lysis of All Common and Hard to Break Cell Types. Mammalian, Yeast, Spores, Mycobacterium, Gram-Positive
   and Negative Bacterium.
Fastest Degradation of DNA/RNA of Any Nuclease on the Market. Clarifies Solution as it Lyses. Cyanase™ can be
   Combined and Stored with Lysis Buffer for Up to 12 Months at 4°C.
Extremely Fast and Easy to Use. From Cells to Completely Clarified Lysate in as Little as 5 Minutes.
No Chemicals or Detergent that Might Affect Downstream Applications. Completely Mechanical Cell Lysis Through
   OmniLyse® Cell Disruptors.
Totally Disposable Columns Run on Convenient Battery Packs(Sold Separately).
시료 파쇄 부분이 더 큰 Cyanase™ Lysis System HL은 효모 등의 크기가 큰 세포의 파쇄 및 시료 양이 많은 경우의 파쇄에
   적합합니다
 
 
Applications:
 
Viral DNA/RNA Isolation
Cell Development Research
• Infectious Disease Studies
Proteomics Research
Any Protocol That Requires Cell Disruption
 
For Downstream Applications Cyanase™ can Easily be Removed From Lysate with any of the available Inactivation Resins.
(Cat#1010,1020 : 아래의 관련페이지 참조) Try To Do That Using Bug Buster with Benzonase®!
 
 
Lysis Protocol:
 
                       
 
1. Add contents of Cyanase™ nuclease tube into lysis buffer before beginning any procedures with the kit.  The
   Cyanase™ will be stable in the lysis buffer at 4°C for up to 1 year.  
2. Resuspend cell pellet up to 1 X 1011 cells in lysis buffer (up to 1 g wet cell paste per ml).  Add 1 M MnSO4 to
   resuspension for a final concentration of 6 mM.  Only add MnSO4 to lysis buffer when you are ready to begin the lysis
   as the MnSO4 is not stable long term at the lysis pH of 8.0.  
3. Pre-wash OmniLyse® cartridge in 500-100 µl of deionized water or 50 mM Tris pH 8.0.  Attach cartridge to
   Bac-Pac™ battery pack via the blue mating connectors, lining up the red arrows.  Attach the luer-lock tubing tip to
   the sample entry port and using the provided syringe, fill the chamber with buffer.  Turn on the battery pack, and
   pass the solution back and forth several times through the cartridge.  Expel all the fluid and turn off battery pack.
4. Insert the tubing tip from the sample port into the sample.  Draw Sample into OmniLyse® cartridge with attached
   syringe, displacing air.
5. Turn on battery pack and slowly draw majority of the remaining sample through the cartridge such that cartridge
   remains filled with sample.  Reverse direction, dispensing the majority of the sample through the cartridge into the
   sample tube.  Continue withdrawing and infusing the sample for 1 minute to 2 minutes, depending on the ease of
   lysis and desired lysis efficiency.  After final removal of sample from syringe, use air to purge the remaining lysis from the
   cartridge.  Turn off battery pack. Cartridges can be reused once on fresh samples within 24 hours if there are no cross
   contamination concerns.
6. Allow the lysis to incubate at room temperature for at least 5 minutes to degrade all remaining nucleic acid
   contamination.  Longer incubations can be performed if desired depending on the final level of nucleic acid removal
   required.
7. Spin down pellet and remove lysis for further processing.  
8. Alternately, Cyanase™ Inactivation resin (Cat#1010, 1020) can be added to the cell lysate after incubation and spun
   as before to remove the Cyanase from the reaction, or the cell lysis can be added to Cyanase™ Inactivation resin
   cartridges (Cat#1015) for high throughput Cyanase™ removal if nucleases are a problem for downstream applications.  
 
 
Product Information: Cyanase™ is active under the following conditions:
 
 
Optimal 
Active
pH
7.5-8.0
6.0-10.0
Temperature
24-37°C
0-50°C
NaCl
0-20 mM
0-100 mM
Mn2+
6-10 mM
1-10 mM
Mg2+
10 mM
1-10 mM
 
Cyanase™ is fully active in DTT up to 100 mM, and most common non-ionic detergents up to 1% including Triton X-100, Tween 20, and Igepal CA630.  Cyanase™ is active in Urea up to 3 M.   Cyanase™ is unaffected by chemical lysate
treatments such as lysozyme or detergent.
Cyanase™ is inhibited by phosphate buffers at 20 mM or higher, and NaCl concentrations of 200 mM or greater.  For
optimal activity, it is recommended to use Mn2+ in place of Mg2+ and avoid any chelating reagents as the enzyme
requires divalent cations for activity.  For pH use greater than 8.3 or less than 7.0, Mg is recommended for maximum
activity due to Mn instability.  It is recommended to add Mn fresh to each reaction for maximal activity.  Do not freeze
Cyanase™ as it will result in a loss of activity of about 10-15%.  See application guide and sample protocols for usage
guidelines.
 
 
Ordering information ;
 
Catalog No.
Description
Size
1100
 Supplied with 25 OmniLyse®Columns, 50 mL of Lysis Buffer, 2,500 U of
 Cyanase and 1 mL of 1 M MnSO4. Usable with Cell Lysates Up to 1 x 108
 Cells
25-50 reactions
1105
 Supplied with 25 OmniLyse®Columns, 50 mL of Lysis Buffer, 2,500 U of
 Cyanase and 1 mL of 1 M MnSO4. Usable with Cell Lysates Up to 1 x 1011
 Cells
25-50 reactions
1111
 Battery Pack - Contains 1 Reusable Battery Pack for OmniLyse® Column
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