FormuMax Scientific
 
작성일 : 16-04-19
[FormuMax Scientific] Superior Efficiency, In vitro Transfection Reagent

 

Trans-Hi™, In vitro DNA Transfection Reagent  

 

 

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Features

• Excellent transfection for single DNA transfection or multi DNA co-transfection

• Rapid onset and long-lasting transfection

• Suitable for very long DNAs

• Simple and easy to use transfection procedure

• Robust and reproducible transfection

• High level of recombinant protein production

• Very cost efficient

• Long shelf-life: 12 months

 

 

Specifications:

 Product type:

In vitro cell transfection reagent

 Applications:

Plasmid transfection, protein overexpression

 Technique:

Polymer-based DNA nanoparticles  

 Cell type:

Established cell lines, primary cells, hard-to-transfect cells

 

Adherent cells and suspension cells

 Sample type:

Plasmid DNA

 Serum compatibility:  

Yes

 Number of transfections:

Up to 660 in 24-well plates with 1.0mL reagent up to 330 transfections in

 

6-well plates

 Shelf-life:

12 months or longer when store refrigerated (2-8°C)

 Shipping condition:

Room temperature

  

 

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Fig. 1. Comparison study of In vitro plasmid DNA transfection of Trans-HiTM and Lipofectamine® 2000 in

HEK293T cells  with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (µL/µg) and

0.5µg DNA per well used for both products in 24 well plate 30 hours post transfection.

 

 

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Fig. 2. Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal

tranfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (µL/µg) (HEK293T cells in 24 well plate 30

hours post transfection, 0.5ug DNA per well).

 

   

 Cell Lines

 Efficiency (% GFP)

 COS-7

 85 - 90%

 Hela

 85 - 90%

 HEK-293

 85-90%

 NIH 3T3

 75%

 A549

 75%

 Hep3D

 65-75%

 SHEP

 65-70%

 CaCo2

 60%

 HepG2

 70%

 SKOV3

 65%

 MCF-7

 65-70%

 3T3-442A

 35-40%

 CHO

 80-90%

Table 1. Broad Transfection Spectrum for Mammalian Cell Types

 

 

Protocols

Trans-Hi™ General Protocol For In Vitro Transfection in Mammalian Cells

Trans-Hi™ Protocol for Transfecting 293 and CHO Suspension Cells

Trans-Hi™ Protocol for Generation of rAAV

Trans-Hi™ Technical Tips For Good In Vitro Transfection

  

 

Ordering information

    

Catalog No.

Product Name

Size

 F90101TH

Trans-Hi™, In vitro Transfection Reagent

0.5ml, 1.0ml & 10 x 1.0ml

 

 

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