Exocell, Inc.
 
작성일 : 16-03-17
[Exocell, Inc.] Urine 중의 Creatinine 정량 Kit

 

Urine 중의 Creatinine 정량 Kit

 


The Creatinine Companion is a chemical assay designed to monitor urinary creatinine in a convenient

microplate format. It is intended as a "companion" for Exocell urinary albumin assays.

 

The assay is based upon the Jaffe' reaction of alkaline picrate with creatinine. Standards or samples are

added to a microplate, and alkaline picrate reagent is added. Absorbance at 500 nm is determined after a 10

minute incubation on the benchtop. Subsequently, an acid solution is added, and absorbance is again

determined after a 5 minute incubation. The difference between these absorbance values is directly

proportional to the the creatinine concentration, and a standard curve is generated from the response to the

standards. Unknown samples are evaluated by comparing response to the standard curve. The assay may

be completed in less than 30 minutes.

 

The assay serves as a normalization procedure for other analytes in urine samples, particularly for urinary

albumin (1,2). It allows for a measure of kidney function based on a spot urine sample rather than the usual

24 hour pool.

 


Kit components

• 96 well microtiter plates

• Acid reagent

• Picrate reagent

• Standard

• NaOH

 


Features

• Specimen Required: Urine, 20 ul

• Assay Range : 10 ~ 100 ug/ml or 1-10 mg/dL

• precision  

: Intra- and interassay precision of samples within the useful range have a C.V.<10% of the mean.

 


References

1. Bennet, P. H. et. al. Screening and management of microalbuminuria in patients with diabetis mellitus.

   Am. J. Kidney Dis. 25:107, 1995.

2. Warram, J. H. et. al. Effect of duration of type 1 diabeties on the prevalence of stages of diabetic

   nephropathy defined by urinary albumin/creatinine ratio. J. Am. Soc. Nephrol. 7:930, 1996.

 



Ordering information


Catalog No.

Product Name

Size

1012 (pdf)

The Creatinine Companion

ELISA KIT

 


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