Tempo Bioscience
 
작성일 : 16-03-08
[Tempo Bioscience] 세포 내 이온농도 등을 형광으로 monitoring 할 수 있는 세포

 

Biosensor, 세포 내의 이온농도 등을 형광으로 monitoring 할 수 있는 세포

세포 내 칼슘 농도 등에 따라 형광을 나타내는 리포터 유전자를 미리 각종 인간 유래 세포에 일시적(transient)으로

도입한 세포입니다.

 

 

TempoVol-iNStem: Cationic Voltage Biosensor Assay using Human Neural Progenitor Cells

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

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TempoVol™, a cationic voltage biosensor responds as a cellular fluorescent reporter (Excitation/Emission:

610nm/625nm) and is incorporated into Tempo's iPSC-derived neural progenitor cells, iNStem. iNStem are

derived from integration-free induced pluripotent stem cell (iPSC) lines under fully defined proprietary neural

induction conditions. TempoCal™-iNStem cells are polarized structures when plated as a monolayer in

culture and express markers such as Vimentin. We've validated iNStem™ cells to be multipotent neural and

glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in vitro

reprogramming conditions. TempoVol™ biosensor is introduced to the cells using proprietary liposome

mediated methods.

 

Applications

• Chemical compound or small molecules testing

• Phenotypic Assays

• Neurotoxicity testing for reporting cationic flux

• Signal transduction pathway modulations

• Ion Channel studies

• Chemical compound or small molecules testing or screening

• For low, medium, or high-throughput applications

 

 

 

TempoMito-iNStem: Mitochondrial Biosensor Assay using Human Neural Progenitors

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

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TempoMito™-iNStem;: Mitochondrial Calcium Biosensor Assay using human Neural Stem Cells. They are

derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary

neural induction condition. TempoMito™-iNStem cells are polarized structures when plated as a monolayer

in culture and express markers such as Vimentin. We've validated iNStem™ cells to be multipotent neural

and glial progenitor cells, as they can be further differentiated into neural and glial cell types under specific in

vitro reprogramming conditions. TempoMito biosensor is introduced to the cells using proprietary liposome

mediated methods and is expressed in the mitochondria (using marker proteins such as COX IV).

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

  클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

Applications

• Neurotoxicity testing for reporting cellular calcium fluctuations

• phenotypic Assays (i.e., mitochondrial toxicity and function)

• Chemical compound or small molecules testing

• Research and development on animal models for human diseases

• Studying neurogenesis, neuronal differentiation and function, and calcium-mediated signal transduction   

  pathways.

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

  클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

 

 

TempoCal-iNStem: Calcium Biosensor Assay using Human Neural Progenitor Cells

 

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

TempoCal™-iNStem are derived from integration-free induced pluripotent stem cell (iPSC) lines under fully

defined proprietary neural induction conditions. TempoCal™-iNStem cells are polarized structures when

plated as a monolayer in culture and express markers such as Vimentin. We've validated iNStem™ cells to

be multipotent neural and glial progenitor cells, as they can be further differentiated into neural and glial cell

types under specific in vitro reprogramming conditions. TempoCal™ biosensor is introduced to the cells

using proprietary liposome mediated methods.

.

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

 

Applications

• Neurotoxicity testing for reporting cellular calcium fluctuations

• Chemical compound or small molecules testing or screening

• Research and Development on animal models

• Studying neurogenesis, neuronal differentiation and function, and calcium-mediated signal transduction

  pathways.

 

 

 

General specifications

 

Long-term Storage

Liquid nitrogen

 

Growth Properties

Adherenet

 

Starting Materials

human dermal/fibroblast cells were reprogrammed using a proprietary foot-print-free technology.

 

Storage

remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw

and use the cells immediately.

      

Product Use

TempoVol™-iNStem cells are intended for basic scientific research and therapeutics development use only.

Not for use in human testing or diagnostic procedures.

 

 

 

Ordering informations

    

Catalog No.

Product Name

Size

SKU 121

TempoVol-iNStem: Cationic Voltage Biosensor Assay

using Human Neural Progenitor Cells

~2x10^6 cells per 1ml

of freezing medium (vial)

SKU 107

TempoMito-iNStem: Mitochondrial Biosensor Assay

using Human Neural Progenitors

~1x10^6 cells per 1ml

of freezing medium (vial)

SKU 104

TempoCal-iNStem: Calcium Biosensor Assay using

Human Neural Progenitor Cells

~1x10^6 cells per 1ml

of freezing medium (vial)

 

   

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