HansaBioMed Life Sciences
 
작성일 : 16-01-13
[HansaBioMed Life Sciences] 2016_monoclonal antibodies for exosome research II
Anti human Alix / TM9SF4 / CD9 antibody


2016_monoclonal antibodies for exosome research II

 

 

Antibodies are an essential tool for basic research, development of diagnostic tests and therapeutics for

human disease. Extracellular vesicles (EVs), including exosomes, express antigens with 3D conformations

and/or post-translational modifications that often differ from the cellular counterpart. For this reason, most of

the antibodies currently available on the market fail to recognize exosome-associated antigenswith sufficient

sensitivity and specificity. In collaboration with Exosomics Siena Spa, HBM has validated a list of polyclonal

and monoclonal antibodies against common (CD63, CD81, ALIX) and disease- specific (cancer  and

neurodegenerative diseases) exosomal markers.

 

 

Features

• All HBM antibodies are tested and validated for exosome antigen reactivity.

• Available in 2 sizes: 50 μg and 100 μg.

 

 

Applications

• Exosome marker detection in different techniques.

• Exosome immunocapture.

 

 

Monoclonal antibodies

 

Anti human ALIX antibody

Description

 

 

 

Alix protein, named also ALG2 interacting protein or PDCD6-interacting protein, is a

cytoplasmic protein that interacts with apoptosis associated-proteins. Alix plays an

active role in exosome biogenesis and it is a useful internal marker for the analysis of

exosomal proteins with western blotting.

Catalgo No. / Size

 

HBM-ALIX-SM1-050 (50 μg)

HBM-ALIX-SM1-100 (100 μg)

Type

Mouse monoclonal unconjugated

Reactivity

Human

Applications

WB, FACS, IF

     클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Western blotting  

1- MM1 (melanoma cell) lysate (20 μg)   

 2- MM1 cells purified exosomes (20 μg)   

3- Plasma healthy donors purified exosomes (20 μg)

FACS

Immunofluorescence staining of HeLa cells.

 

           

Anti human TM9SF4 antibody

Description

 

 

 

 

 

 

TM9SF4 (TUCAP1) is a newly discovered tumor-associated protein of unknown function

that belongs to the Trans-Membrane 9 Superfamily (TM9SF). These proteins are

characterized by the presence of a large variable extracellular N-terminal domain

followed by nine putative transmembrane domains and a conserved C-terminal domain.

TM9SF4 is mainly expressed on exosomes derived from tumor tissue. HBM offers three

differentmonoclonal antibodies that recognize the protein on both exosomes and

cell lysates.

Catalgo No. / Size

 

HBM-SF4-PM-050 (50 μg)

HBM-SF4-PM-100 (100 μg)

Type

Mouse monoclonal unconjugated

Reactivity

Human

Applications

WB, FACS, IP

 

 클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Western blotting  

1- 20μg of whole lysate of melanoma cell line   

2- 20μg whole lysate of MM1 cell supernanant

purified exosomes 

3- 20μg of purified exosomes from human plasma of

healthy donors (PEP)

FACS

Staining of TM9SF4 on MM1 purified exosomes vs

human plasma exosomes (PEP) demonstrates the

predominant TM9SF4 expression in exosomes

derived from tumoral source

                          

Anti mouse CD9 antibody

Description

 

 

 

 

Anti-CD9 recognizes an human 24-kiloDalton (kDa) single-chain cell-surface

glycoprotein (p24)belonging to the tetraspanin family. CD9 has a very broad tissue

distribution and is abundant on exosome membranes. HBM off ers a monoclonal anti-

CD9 antibody unconjugated reactive against mouse antigen and able to identify CD9 in

mouse cell of biofl uids derived exosomes.

Catalgo No. / Size

 

HBM-CD9M-050 (50 μg)

HBM-CD9M-100 (100 μg)

Type

Mouse monoclonal unconjugated

Reactivity

Mouse

Applications

WB, ELISA, FACS, IP, IHC

 

     클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

         클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Western blotting  

CD9 staining of B16F10 mouse cell  purified exosomes

 

Western blotting

1- 20μg of whole lysate of  B16F10 cell supernatant

purified exosomes

2-20μg of exosomes isolated from mouse plasma

                   

           

 

Ordering informations

     

Catalog No.

Description

size

HBM-ALIX-(050/100)

Anti human Alix antibody

50 μg / 100 μg

HBM-SF4-(050/100)

Anti human TM9SF4 antibody

50 μg / 100 μg

HBM-CD9M-(050/100)

Anti mouse CD9 antibody

50 μg / 100 μg

    

 

HansaBioMed 2016 catalog (PDF)

 

 

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